Nanos3 maintains the germ cell lineage in the mouse by suppressing both Bax-dependent and -independent apoptotic pathways
| Autor: | Hitomi Suzuki, Makoto Kiso, Masayuki Tsuda, Yumiko Saga |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Male
endocrine system Programmed cell death Somatic cell Cell Survival Knockin mice Cre recombinase Apoptosis Mice Transgenic Biology Mice Oogenesis Cell Movement Genes Reporter Lineage analysis Testis medicine Animals Cell Lineage Spermatogenesis Molecular Biology Germ plasm bcl-2-Associated X Protein Mice Knockout urogenital system Caspase 3 RNA-Binding Proteins Embryo Cell Biology medicine.disease Molecular biology Cell biology Mice Inbred C57BL medicine.anatomical_structure Germ Cells Primordial germ cell (PGC) NANOS3 Gene embryonic structures Gene Targeting Female Germ cell tumors Germ line development Germ cell Developmental Biology |
| Zdroj: | Developmental biology. 318(1) |
| ISSN: | 1095-564X |
| Popis: | Cell death in the germ line is controlled by both positive and negative mechanisms that maintain the appropriate number of germ cells and that prevent the possible formation of germ cell tumors. In the mouse embryo, Steel/c-Kit signaling is required to prevent migrating primordial germ cells (PGCs) from undergoing Bax-dependent apoptosis. In our current study, we show that migrating PGCs also undergo apoptosis in Nanos3-null embryos. We assessed whether the Bax-dependent apoptotic pathway is responsible for this cell death by knocking out the Bax gene together with the Nanos3 gene. Differing from Steel-null embryos, however, the Bax elimination did not completely rescue PGC apoptosis in Nanos3-null embryos, and only a portion of the PGCs survived in the double knockout embryo. We further established a mouse line, Nanos3-Cre-pA, to undertake lineage analysis and our results indicate that most of the Nanos3-null PGCs die rather than differentiate into somatic cells, irrespective of the presence or absence of Bax. In addition, a small number of surviving PGCs in Nanos3/Bax-null mice are maintained and differentiate as male and female germ cells in the adult gonads. Our findings thus suggest that heterogeneity exists in the PGC populations and that Nanos3 maintains the germ cell lineage by suppressing both Bax-dependent and Bax-independent apoptotic pathways. |
| Databáze: | OpenAIRE |
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