Radiation retards muscle differentiation but does not affect osteoblastic differentiation induced by bone morphogenetic protein-2 in C2C12 myoblasts
| Autor: | S. Ikeda, R. Hachisu, Akira Yamaguchi, Y.-H. Gao, T. Okano |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
Cellular differentiation Osteocalcin Bone Morphogenetic Protein 2 Biology Myosins Bone morphogenetic protein Bone morphogenetic protein 2 Cell Line Mice Transforming Growth Factor beta Internal medicine Myosin medicine Myocyte Animals Humans Radiology Nuclear Medicine and imaging RNA Messenger Muscle Skeletal Osteoblasts Radiological and Ultrasound Technology Myosin Heavy Chains Myogenesis Osteoblast Cell Differentiation musculoskeletal system Alkaline Phosphatase Recombinant Proteins Cell biology Endocrinology medicine.anatomical_structure Bone Morphogenetic Proteins Myogenin tissues C2C12 |
| Zdroj: | International journal of radiation biology. 76(3) |
| ISSN: | 0955-3002 |
| Popis: | Effects of radiation on the differentiation of C2C12 myoblasts were studied using cell cultures in which the cells differentiated into either muscle cells or osteoblasts.A mouse skeletal myoblast cell line, C2C12, was cultured and irradiated at confluence. Muscle differentiation was estimated by counting the number of myosin heavy chain (MHC)-positive multinucleated myotubes. Osteoblast differentiation was analysed by measuring alkaline phosphatase (ALP) activity and osteocalcin production in C2C12 cells treated with recombinant human bone morphogenetic protein-2 (rhBMP-2).Myotube formation in C2C12 cells was inhibited by 2 and 4 Gy irradiation, but recovered to non-irradiated control levels following further culture. In irradiated cultures, the number of MHC-positive mononucleated cells increased significantly. Irradiation did not, however, alter the induction of osteoblastic phenotypes induced by rhBMP-2 in C2C12. Irradiation also did not affect the expression of MyoD mRNA and myogenin mRNA in myogenic differentiation and induction of osteocalcin mRNA in osteoblastic differentiation.During differentiation of C2C12 cells, myotube formation was delayed after irradiation, while induction of osteoblasts with BMP-2 was unaffected. |
| Databáze: | OpenAIRE |
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