Quantitative proliferation dynamics and random chromosome segregation of hair follicle stem cells
| Autor: | Rajat Bansal, Ying V. Zhang, Tudorita Tumbar, Jayhun Lee, Sanjeev K. Waghmare, David J. McDermitt |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Cell division Pyridines Recombinant Fusion Proteins Green Fluorescent Proteins Immortal DNA strand hypothesis General Biochemistry Genetics and Molecular Biology Article Chromosome segregation Histones Mice Chromosome Segregation medicine Compartment (development) Animals Molecular Biology Cell Proliferation Microscopy Confocal General Immunology and Microbiology biology Reverse Transcriptase Polymerase Chain Reaction General Neuroscience Stem Cells Chromosome Hair follicle Flow Cytometry Molecular biology Cell biology medicine.anatomical_structure Histone Bromodeoxyuridine Microscopy Fluorescence biology.protein Female Stem cell Hair Follicle Cell Division |
| Zdroj: | The EMBO journal. 27(9) |
| ISSN: | 1460-2075 |
| Popis: | Regulation of stem cell (SC) proliferation is central to tissue homoeostasis, injury repair, and cancer development. Accumulation of replication errors in SCs is limited by either infrequent division and/or by chromosome sorting to retain preferentially the oldest ‘immortal’ DNA strand. The frequency of SC divisions and the chromosome‐sorting phenomenon are difficult to examine accurately with existing methods. To address this question, we developed a strategy to count divisions of hair follicle (HF) SCs over time, and provide the first quantitative proliferation history of a tissue SC during its normal homoeostasis. We uncovered an unexpectedly high cellular turnover in the SC compartment in one round of activation. Our study provides quantitative data in support of the long‐standing infrequent SC division model, and shows that HF SCs do not retain the older DNA strands or sort their chromosome. This new ability to count divisions in vivo has relevance for obtaining basic knowledge of tissue kinetics. |
| Databáze: | OpenAIRE |
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