Islet damage during isolation as assessed by miRNAs and the correlation of miRNA levels with posttransplantation outcome in islet autotransplantation

Autor: Carly M. Darden, Bashoo Naziruddin, Prathab Balaji Saravanan, Mazhar A. Kanak, Michael C. Lawrence, Charles A. Chang, Gumpei Yoshimatsu
Rok vydání: 2018
Předmět:
Adult
Blood Glucose
Graft Rejection
Male
endocrine system
medicine.medical_specialty
medicine.medical_treatment
Islets of Langerhans Transplantation
Endocrine System
Cell Separation
030230 surgery
Transplantation
Autologous
Islets of Langerhans
03 medical and health sciences
Postoperative Complications
0302 clinical medicine
Risk Factors
Internal medicine
Diabetes mellitus
medicine
Humans
Insulin
Immunology and Allergy
Endocrine system
Pharmacology (medical)
Transplantation
geography
geography.geographical_feature_category
C-Peptide
business.industry
Pancreatic islets
Graft Survival
medicine.disease
Islet
Autotransplantation
MicroRNAs
Diabetes Mellitus
Type 1
Treatment Outcome
Endocrinology
medicine.anatomical_structure
Biomarker (medicine)
Female
030211 gastroenterology & hepatology
Hemoglobin
business
Follow-Up Studies
Zdroj: American Journal of Transplantation. 18:982-989
ISSN: 1600-6135
Popis: High-quality pancreatic islets are essential for better posttransplantation endocrine function in total pancreatectomy with islet autotransplantation (TPIAT), yet stress during the isolation process affects quality and yield. We analyzed islet-enriched microRNAs (miRNAs) -375 and -200c released during isolation to assess damage and correlated the data with posttransplantation endocrine function. The absolute concentration of miR-375, miR-200c, and C-peptide was measured in various islet isolation steps, including digestion, dilution, recombination, purification, and bagging, in 12 cases of TPIAT. Posttransplantation glycemic control was monitored through C-peptide, hemoglobin A1c , insulin requirement, and SUITO index. The amount of miR-375 released was significantly higher during enzymatic digestion followed by the islet bagging (P < .001). Mir-200c mirrored these changes, albeit at lower concentrations. In contrast, the C-peptide amount was significantly higher in the purification and bagging steps (P < .001). Lower amounts of miR-375 were associated with a lower 6-month insulin requirement (P = .01) and lower hemoglobin A1c (P = .04). Measurement of the absolute quantity of miRNA-375 and -200c released during islet isolation is a useful tool to assess islet damage. The quantity of released miRNA is indicative of posttransplantation endocrine function in TPIAT patients.
Databáze: OpenAIRE
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