Prognostic Value of JC Virus Load in Cerebrospinal Fluid of Patients with Progressive Multifocal Leukoencephalopathy
| Autor: | Eric Marchadier, Annie Lannuzel, Cécile Goujard, Elisabeth Dussaix, Marie Pierre Ferey, Jacques Gasnault, Amina Karaterki, Yassine Taoufik, Jean François Delfraissy |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Cellular immunity Pathology medicine.medical_specialty AIDS Dementia Complex viruses Organophosphonates JC virus Biology medicine.disease_cause Antiviral Agents Polymerase Chain Reaction Statistics Nonparametric Virus Cytosine chemistry.chemical_compound Organophosphorus Compounds Cerebrospinal fluid Predictive Value of Tests medicine Humans Immunology and Allergy DNA Primers Slow virus AIDS-Related Opportunistic Infections Progressive multifocal leukoencephalopathy Papillomavirus Infections virus diseases Viral Load Prognosis medicine.disease JC Virus Virology CD4 Lymphocyte Count Tumor Virus Infections Infectious Diseases chemistry DNA Viral HIV-1 Female DNA Probes Viral load Cidofovir |
| Zdroj: | The Journal of Infectious Diseases. 178:1816-1820 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1086/314496 |
| Popis: | JC virus (JCV) load was determined by using quantitative polymerase chain reaction in cerebrospinal fluid (CSF) of 12 patients with AIDS-associated progressive multifocal leukoencephalopathy (PML) and compared with clinical outcome. JCV loads varied widely (3‐7 log10 JCV equivalents/mL of CSF) and were apparently not related to absolute CD4 cell counts or CSF and plasma human immunodeficiency virus type 1 loads. A significant correlation was observed between JCV load and survival time (Spearman’s rank correlation, 20.83; ). Moreover, CSF JCV load decreased and then became undetectable in P ! .01 1 PML patient receiving cidofovir treatment, and this was associated with clinical improvement. These results show that CSF JCV load may be useful as a prognostic parameter and in monitoring the effectiveness of anti-JCV therapies in PML patients. The human polyomavirus JC virus (JCV) is the causative agent of progressive multifocal leukoencephalopathy (PML), a fatal demyelinating neurodegenerative disease associated with impaired cellular immunity [1]. The prevalence of PML in AIDS patients is »4% [1]. PML results from lytic infection of oligodendrocytes, leading to foci of demyelinization in the cerebral white matter [1]. JCV DNA detection in cerebrospinal fluid (CSF) is a reliable diagnostic marker of PML, particularly when combined with a typical magnetic resonance imaging pattern [1‐5]. However, most available JCV polymerase chain reaction (PCR) methods provide no information on virus load. Here, by using quantitative PCR, we investigated JCV load in CSF of AIDS-associated PML patients. |
| Databáze: | OpenAIRE |
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