Characterization and promoter analysis of the mouse gene for transcription factor Sp4
| Autor: | Monika Mangold, Guntram Suske, Kailai Sun, Kazunari K. Yokoyama, Christian Geltinger, Jun Song, Ichiro Kanazawa |
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| Rok vydání: | 2001 |
| Předmět: |
Transcription
Genetic Sp1 Transcription Factor Recombinant Fusion Proteins TATA box Molecular Sequence Data Response element CAAT box Sp4 Transcription Factor Biology Cell Line Mice Upstream activating sequence Epigenetics of physical exercise Sequence Homology Nucleic Acid Genetics Animals Humans Amino Acid Sequence Luciferases Promoter Regions Genetic Enhancer Sequence Deletion Base Sequence Promoter DNA Sequence Analysis DNA General Medicine Molecular biology DNA-Binding Proteins Sp3 Transcription Factor Gene Expression Regulation TAF2 Sequence Alignment Protein Binding Transcription Factors |
| Zdroj: | Gene. 264:19-27 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/s0378-1119(01)00328-6 |
| Popis: | Transcription factor Sp4 is a member of the Sp1 family. It functions differently from other members of this family, such as Sp1 and Sp3, and the gene for Sp4 is transcribed in a tissue-specific manner. Recent studies in mice suggest that Sp4 might play an important role in growth, viability, and male fertility. We report here the isolation and characterization of the gene for Sp4 from a mouse genomic library. The mouse gene for Sp4 was about 80 kb in length and it consisted of six exons and five introns. The promoter was found in a CpG island and had a high G+C content. The proximal promoter contained multiple putative binding sites for the transcription factors Sp1 and MAZ but lacked a consensus TATA box. Multiple sites for the initiation of transcription were mapped in a GC-rich region from 286 bp to 211 bp upstream of the ATG triplet at the site of initiation of translation, and all of the sites were either C or G. Transfection experiments and deletion analysis allowed us to localize the promoter to a region that was no more than 93 bp upstream from the first site of initiation of transcription. We also found that ectopic expression of Sp1 and of MAZ, but not of Sp3, suppressed expression of the Sp4 promoter in a dose-dependent manner. |
| Databáze: | OpenAIRE |
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