Priming of allo-HLA-DP-specific reactivity from the naive T cell compartment is not exclusively mediated by professional antigen-presenting cells
| Autor: | Aicha Laghmouchi, J.H. Frederik Falkenburg, Inge Jedema, Conny Hoogstraten |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
CD4-Positive T-Lymphocytes
HLA-DP Antigens Naive T cell Priming (immunology) Antigen-Presenting Cells Graft vs Host Disease Graft vs Leukemia Effect Graft-versus-host disease 03 medical and health sciences 0302 clinical medicine Immune system Allogeneic HSCT Medicine Humans Antigen-presenting cell Transplantation business.industry HLA-DP Hematopoietic Stem Cell Transplantation Hematology medicine.disease T cell response medicine.anatomical_structure Graft-versus-leukemia effect 030220 oncology & carcinogenesis Immunology Stem cell business Memory T cell 030215 immunology |
| Zdroj: | Biology of Blood and Marrow Transplantation, 26(7), 1257-1265. ELSEVIER SCIENCE INC |
| Popis: | Allogeneic (allo) stem cell transplantation is applied to patients suffering from hematologic malignancies to replace the diseased hematopoietic system with cells derived from a donor stem cell graft. The majority of 10/10matched unrelated donors are HLA-DP-mismatched, and this may result in varying degrees of the graft-versusleukemia (GVL) effect with or without the occurrence of graft-versus-host disease (GVHD). Allo-HLA-reactive T cells are commonly present in the donor T cell repertoire, and thus a very profound alloreactive immune response can be provoked in the HLA-DP-mismatched setting. The magnitude and the diversity of the allo-HLA-DP-specific immune response likely dictates the balance between the occurrence of GVL and/or GVHD after transplantation. To understand the nature of the allo-HLA-DP-specific immune response provoked under different stimulatory conditions, immune responses were induced from both the naive and memory T cell compartments using either HLA-DP-mismatched professional antigen-presenting cells (APCs) (monocyte-derived dendritic cells [allo-DCs]) or HLA-DP-mismatched nonprofessional APCs (skin-derived fibroblasts [allo-fibroblasts]) as stimulator cells. In this study, we observed that allo-HLA-DP-reactive T cells could be provoked from both the naive and memory compartments by both types of APCs. However, the magnitude of the allo-HLA-DP-specific immune response was greater when stimulation was performed with allo-DCs. Moreover, we found that the frequency of allo-HLA-DPreactive T cells was greater in the naive T cell compartment compared with the memory T cell compartment, but we observed a comparable lineage specificity of these allo-HLA-DP-specific reactivities. Overall, the data from this study illustrate that the presence of professional APCs of recipient origin will mostly dictate the magnitude of the allo-HLA-DP-specific immune response derived from both the naive and memory T cell compartments, but does not exclusively mediate the induction of these immune responses. (C) 2020 American Society for Transplantation and Cellular Therapy. Published by Elsevier Inc. |
| Databáze: | OpenAIRE |
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