Ethanolamines permeate slowly across human skin ex vivo, but cause severe skin irritation at low concentrations

Autor: Gregory Plateel, Philipp Spring, Nancy B. Hopf, Aurélie Berthet
Rok vydání: 2019
Předmět:
Zdroj: Archives of Toxicology. 93:2555-2564
ISSN: 1432-0738
0340-5761
DOI: 10.1007/s00204-019-02542-2
Popis: Skin exposures are common during cleaning activities, and may contribute to the overall body burden. Cleaning products may contain irritants such as monoethanolamine (MEA) and diethanol amine (DEA). The significance of the skin exposure route is unknown, as no estimates for MEA skin permeation are available. We used in vitro flow-through diffusion cells with excised fresh human skin to measure skin permeation, and assessed skin damage with histological methods. MEA(aq) by itself (2%) or as a constituent in cleaning products (0.25% working solution) did not permeate after 1 h or 24 h of exposure. MEA(aq) (10%) did not permeate skin after 1 h but after 24 h with a delay (Tlag; 7 h) and a moderate permeation rate (J; 26.6 μg/cm2/h). MEA permeation rate was 20-fold greater (544 μg/cm2/h) and ¼ of the time lag (1.5 h) when applied as undiluted cleaning product (13% MEA) compared to 10% MEA(aq). DEA in cleaning products did not permeate skin after 24 h. MEA and DEA produced skin irritations at low concentrations (1% MEA) and severe skin irritations when tested as a constituent in cleaning products. Absorption increased from 0 to 3% after 24 h to 14–29% after 88 h of MEA exposure, and is likely explained by the increased damage of the skin barrier. Limitations of this study are the low number of skin donors (N = 5) available. Our results demonstrate that topically applied MEA permeates across human skin relatively slowly and not below 5% while relatively extensively as a constituent of a commercial cleaning product.
Databáze: OpenAIRE
Externí odkaz: