Solid phase extraction and HPLC determination of spiramycin in plasma and vitreous concentrations
| Autor: | M. A. Le Bot, C. Riche, F. Mimouri, Carlhant D, J. Colin, F. Berthou, Y. Guedes |
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| Rok vydání: | 1989 |
| Předmět: |
Pharmacology
Detection limit Chromatography Vitreous humour Chemistry Computers Coefficient of variation Clinical Biochemistry Spiramycin General Medicine Biochemistry High-performance liquid chromatography Leucomycins Analytical Chemistry Vitreous Body Cartridge Pharmacokinetics Drug Discovery medicine Humans Solid phase extraction Ophthalmic Solutions Molecular Biology Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Biomedical chromatography : BMC. 3(1) |
| ISSN: | 0269-3879 |
| Popis: | A method for extracting spiramycin by an octadecylsilica cartridge is described for plasma or vitreous samples. The macrolide antibiotic is then measured by reversed-phase HPLC with UV detection. The limit of detection is estimated to be 50 ng/mL. The coefficient of variation for the procedure is 6.1% and 5.2% for the range of concentrations 0.2 micrograms/mL and 10 micrograms/mL respectively. By this method, pharmacokinetic profiles were performed for five adult patients. Spiramycin could be accurately measured in the vitreous humour, allowing the determination of antibiotic at its site of action. |
| Databáze: | OpenAIRE |
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