Bovine granulocyte chemotactic protein-2 is secreted by the endometrium in response to interferon-tau (IFN-τ)
| Autor: | David J. Perry, Greg A. Johnson, Vern D. Dooley, Kathy J. Austin, M. Glaucia Teixeira, Brian R. Francis, Thomas R. Hansen |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Antiserum
medicine.medical_specialty Chemokine biology Endocrinology Diabetes and Metabolism medicine.medical_treatment Endometrium Molecular biology Interferon tau Endocrinology medicine.anatomical_structure Cytokine Polyclonal antibodies Internal medicine medicine biology.protein Peptide sequence Keyhole limpet hemocyanin |
| Zdroj: | Endocrine. 6(1) |
| ISSN: | 1355-008X |
| Popis: | Interferon-tau (IFN-τ) is secreted by the bovine conceptus and may regulate synthesis of uterine endometrial cytokines to provide an environment that is conducive to embryo development and implantation. Interferon-τ stimulates secretion of an 8-kDa uterine protein (P8) in the cow. P8 was purified, digested to yield internal peptides, and partially sequenced to determine identity. Two internal peptides had 100% (13-mer) and 92% (12-mer) amino acid sequence identity with bovine granulocyte chemotactic protein-2 (bGCP-2). Bovine GCP-2 is an α-chemokine that acts primarily as a potent chemoattractant for granulocyte cells of the immune system. A peptide was synthesized based on a region of bGCP-2 that overlapped with a P8 peptide amino acid sequence, coupled to keyhole limpet hemocyanin, and used to generate high titer polyclonal antiserum in sheep. Western blots revealed that bGCP-2 was not released by endometrium from day 14 nonpregnant cows, but was released in response to 25 nM IFN-τ (p |
| Databáze: | OpenAIRE |
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