Stearic acid methyl ester promotes migration of mesenchymal stem cells and accelerates cartilage defect repair
| Autor: | Junlang Zhu, Yamei Liu, Hongtai Chen, Gang Li, Chen Chen, Lijuan Yu, Bin Wang, Dongfeng Chen, Yiwen Luo, Liuchao Hu, Xican Li, Liangliang Xu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
030203 arthritis & rheumatology
0301 basic medicine Small interfering RNA lcsh:Diseases of the musculoskeletal system Chemistry Cartilage Mesenchymal stem cell Cell Chondrogenesis Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure In vivo Vav1 medicine Mesenchymal stem cells Original Article Orthopedics and Sports Medicine MTT assay lcsh:RC925-935 Stearic acid methyl ester Homing (hematopoietic) |
| Zdroj: | Journal of Orthopaedic Translation, Vol 22, Iss, Pp 81-91 (2020) Journal of Orthopaedic Translation |
| ISSN: | 2214-031X |
| Popis: | Background Mesenchymal stem cells (MSCs) can be easily expanded without losing the ability of multilineage differentiation, including oesteogenic, chondrogenic and adipogenic differentiation. These characters make MSCs a promising cell resource for cartilage defect repair. MSCs could be recruited by inflammatory stimulation, then home to the injury tissues. However, its capacity of homing is extremely limited. Thus, it has become extremely necessary to develop an agent or a method, which can be used to enhance the efficiency of MSCs homing. This study investigates the effect of stearic acid methyl ester (SAME) on MSCs mobilisation and cartilage regeneration. Methods MSCs were isolated from femurs of Sprague-Dawley (SD) rats. MTT assay was used to detect effect of SAME on viability of MSCs. Transwell assay and wound healing assay were used to detect effect of SAME on migration of MSCs. RNA-seq, quantitative real-time PCR and western blot were performed to analyze the expression of RNAs and proteins. Colony forming assay and flow cytometry were used to evaluate the effect of SAME on MSCs mobilisation in vivo. A rat cartilage defect model was created to evaluate the effect of SAME on cartilage regeneration. Results We found that SAME could promote the migration of MSCs. Interestingly, we found SAME significantly increased the expression levels of Vav1 in MSCs. On the other hand, the enhanced migration ability of MSCs induced by SAME was retarded by Vav1 small interfering RNA (siRNA) and Rho-associated protein kinase 2 (ROCK2) inhibitor. In addition, we also checked the effect of SAME on mobilisation of MSCs in vivo. The results showed that SAME increased the number of MSCs in peripheral blood and enhanced the capacity of colony formation. Finally, using a cartilage defect model in rats, we found SAME could improve cartilage repair. Conclusion Our study demonstrates that SAME can enhance MSCs migration ability mainly through the Vav1/ROCK2 signaling pathway, which could contribute to the accelerated cartilage regeneration. The translational potential of this article These findings provide evidence that SAME could be used as a therapeutic reagent for MSCs mobilisation and cartilage regeneration. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|