Adenosine triphosphate in tarantula spider venoms and its synergistic effect with the venom toxin
| Autor: | D.E. Howell, Tak K. Chan, Collis R. Geren, George V. Odell |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Spider Venoms
Venom Biology Toxicology medicine.disease_cause complex mixtures Median lethal dose Lethal Dose 50 Mice chemistry.chemical_compound Adenosine Triphosphate medicine Animals Luciferase Arthropod Venoms Toxins Biological Toxin Drug Synergism Chromatography Ion Exchange Adenosine Biochemistry chemistry Toxicity Chromatography Gel Adenosine triphosphate medicine.drug |
| Zdroj: | Toxicon. 13:61-66 |
| ISSN: | 0041-0101 |
| DOI: | 10.1016/0041-0101(75)90159-2 |
| Popis: | The presence of adenosine 5′-triphosphate, adenosine 5′-diphosphate and adenosine 5′-phosphate in the venoms of the tarantula spiders, Dugesiella hentzi and Aphonopelma sp. have been identified by ion exchange chromatography, thin-layer chromatography and u.v. absorption spectrometry. Adenosine 5′-triphosphate, the major nucleotide component, was present at a concentration of 28·1 μg per μl venom in D. hentzi venom, and 56·6 μg per μl venom in Aphonopelma sp. venom as assayed by the firefly luciferase method. Toxicity studies indicated that ATP had synergistic toxic effects with the major venom toxin of the D. hentzi venom. The ld 50 value of the venom toxin in mice was significantly diminished when ATP was added. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|