Entomopathogenic Fungi Biomass Production and Extracellular Biosynthesis of Silver Nanoparticles for Bioinsecticide Action
| Autor: | Marcelo da Costa Mendonça, Matheus G. de Jesus Seabra, Patrícia Severino, Eliana B. Souto, Tárcio Souza Santos, Eliana M. dos Passos |
|---|---|
| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine silver nanoparticles Microorganism Biomass Virulence Biosynthesis 7. Clean energy 01 natural sciences lcsh:Technology Silver nanoparticle Conidium lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound General Materials Science Food science Instrumentation lcsh:QH301-705.5 2. Zero hunger Fluid Flow and Transfer Processes Science & Technology lcsh:T Process Chemistry and Technology fungi General Engineering entomopathogenic fungi lcsh:QC1-999 Bioinsecticide Computer Science Applications 010602 entomology Silver nitrate Entomopathogenic fungi 030104 developmental biology chemistry lcsh:Biology (General) lcsh:QD1-999 13. Climate action lcsh:TA1-2040 Entomopathogenic fungus bioinsecticide Silver nanoparticles biosynthesis lcsh:Engineering (General). Civil engineering (General) lcsh:Physics |
| Zdroj: | Applied Sciences, Vol 11, Iss 2465, p 2465 (2021) Applied Sciences Volume 11 Issue 6 Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 2076-3417 |
| Popis: | Entomopathogenic fungi are microbial agents of insect control in nature. They have been used as biologic strategies to manage insect invasion; however, the challenge is to maintain their shelf life and viability when exposed to high temperatures, ultraviolet radiation, and humidity. Synthesized silver nanoparticles (AgNPs) from fungal extracellular enzymes are an alternative using these microorganisms to obtain nanoparticles with insecticidal action. The present study evaluates the biomass production and the potential to synthesize silver nanoparticles using entomopathogenic fungi isolates. Sixteen isolates of entomopathogenic fungi were used in this study. The fungi pathogenicity and virulence were evaluated using the insect model Tenebrio molitor, at a concentration of 5 × 106 conidia/mL. The fungal biomass was produced in a liquid medium, dried, and weighed. The synthesis of silver nanoparticles was performed with aqueous extracts of the entomopathogenic fungi and silver nitrate solution (1 mM), following characterization by a UV/vis spectrophotometer, mean size, and polydispersity index. The results showed a significant variation in pathogenicity, virulence, and biomass production among the evaluated fungi isolates; however, only one of the isolates did not have the potential to synthesize silver nanoparticles. Pearsons correlation showed significant correlation values only between virulence × biosynthesis potential and biomass production × biosynthesis potential, both with negative values, indicating an inverse correlation. Thus, AgNPs with entomopathogenic fungus extract can produce an innovative bioinsecticide product using a green production process. This work was supported by the Fundação de Amparo à Pesquisa do Estado de Sergipe (FAPITEC), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, #443238/2014-6, #470388/2014-5) and Banco do Nordeste (FUNDECI/2017.0014), the Portuguese Science and Technology Foundation (FCT/MCT), and from European Funds (PRODER/COMPETE) for the project UIDB/04469/2020 (strategic fund), co-financed by FEDER, under the Partnership Agreement PT2020. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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