Fluorescence anisotropy studies on the Hoechst 33258-DNA interaction: the solvent effect
Autor: | Hasmik R. Sargsyan, Heghine H. Ghazoyan, Shiraz A. Markarian, Gohar A. Shahinyan, Karen Yu. Amirbekyan |
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Rok vydání: | 2020 |
Předmět: |
0303 health sciences
Molecular interactions Dimethyl sulfoxide 030303 biophysics Dna interaction Fluorescence Polarization General Medicine DNA 03 medical and health sciences chemistry.chemical_compound Spectrometry Fluorescence chemistry Structural Biology Biophysics Bisbenzimidazole Solvents Thermodynamics Solvent effects Molecular Biology Fluorescence anisotropy Minor groove |
Zdroj: | Journal of biomolecular structuredynamics. 39(13) |
ISSN: | 1538-0254 |
Popis: | Fluorescence anisotropy method was applied to characterize the interactions of DNA minor groove binder Hoechst 33258 with different solvents without and in the presence of DNA. It is important to study the interaction of small molecules with DNA for the purpose of better understanding the mechanism of their action, as well as to design novel and more effective compounds. Spectroscopic study of the ligand in different binary mixed solvents containing DMSO, alcohols and buffer was carried out. Studies were performed without and in the presence of DNA. Fluorescence anisotropy studies reveal the characteristics of Hoechst 33258 in different mixed solvents. The results show the strong dependence of the anisotropy of Hoechst 33258 on solvent content, viscosity and intermolecular interactions. Communicated by Ramaswamy H. Sarma. |
Databáze: | OpenAIRE |
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