A new method for permeabilization of cultured cells without cell damage
| Autor: | Naoaki Shimizu, Yutaka Kawazoe |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Cell Membrane Permeability
Cell Survival Cell Acrylic Resins Pharmaceutical Science Antineoplastic Agents Biology Bleomycin Mice chemistry.chemical_compound medicine Animals Leukemia L1210 Cytotoxicity Cell damage Cells Cultured Pharmacology Lucifer yellow Antibiotics Antineoplastic Polyacrylic acid General Medicine Isoquinolines medicine.disease Molecular biology In vitro Membrane medicine.anatomical_structure chemistry Cell culture |
| Zdroj: | Biological and Pharmaceutical Bulletin. 19:484-486 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.19.484 |
| Popis: | Transient permeabilization was readily achieved by vortex-stirring cultured mammalian cells for a few seconds in the presence of 1 to 10 micrograms/ml of high molecular weight polyacrylic acid (PAA). This was demonstrated by an appreciable uptake by murine leukemia L1210 cells of Lucifer Yellow (LY), which is known to be a non-permeant except under conditions in which micropores are induced in the plasma membrane. In addition, the cytotoxicity of bleomycin (BLM), a very poor permeant, was markedly enhanced by vortex-stirring with PAA. Scope and limitation of this permeabilization procedure is preliminarily described. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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