Oxygen and Glucose Levels in Cell Culture Media Determine Resveratrol’s Effects on Growth, Hydrogen Peroxide Production, and Mitochondrial Dynamics
| Autor: | Fereshteh Moradi, Andrew J. F. Valente, Jeffrey A. Stuart, Joao Fonseca |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Antioxidant Physiology medicine.medical_treatment Clinical Biochemistry resveratrol Mitochondrion Resveratrol Biochemistry Article 03 medical and health sciences chemistry.chemical_compound medicine Hydrogen peroxide Molecular Biology reactive oxygen species chemistry.chemical_classification Reactive oxygen species Chemistry Cell growth lcsh:RM1-950 food and beverages Cell Biology Metabolism glycolysis Warburg mitochondrial dynamics mitochondria lcsh:Therapeutics. Pharmacology 030104 developmental biology Cell culture metabolism hormones hormone substitutes and hormone antagonists |
| Zdroj: | Antioxidants, Vol 7, Iss 11, p 157 (2018) Antioxidants Volume 7 Issue 11 |
| ISSN: | 2076-3921 |
| Popis: | Resveratrol is a plant-derived polyphenol that has been widely studied for its putative health promoting effects. Many of those studies have been conducted in cell culture, in supra-physiological levels of oxygen and glucose. Resveratrol interacts with reactive oxygen species (ROS) as an antioxidant or pro-oxidant. Resveratrol affects the expression and activities of ROS-producing enzymes and organelles. It is therefore important to consider how cell culture conditions might determine the effects of resveratrol on cultured cells. We determined the effects of resveratrol on cell growth, hydrogen peroxide production, and mitochondrial network characteristics in C2C12 mouse myoblasts and PC3 human prostate cancer cells under conditions of physiological (5%) and supra-physiological (18%) oxygen, and normo- (5 mM) and hyper-glycemia (25 mM). Interestingly, most effects of resveratrol on the parameters measured here were dependent upon prevailing oxygen and glucose levels during the experiment. Many of the effects of resveratrol on cell growth, hydrogen peroxide production, and mitochondrial network characteristics that were seen in 25 mM glucose and/or 18% oxygen were absent under the physiologically relevant conditions of 5 mM glucose with 5% oxygen. These findings emphasize the importance of using physiologically meaningful starting conditions for cell-culture experiments with resveratrol and indeed any manipulation affecting ROS metabolism and mitochondria. |
| Databáze: | OpenAIRE |
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