The Applicability of Species- and Trichothecene-Specific Primers in Monitoring the Fusarium graminearum Species Complex and Its Impact on the Surveillance of Fusarium Head Blight in Winter Wheat in Serbia
| Autor: | Sanja Mikić, Vesna Župunski, Branka Orbović, Mirjana Lalošević, Radivoje Jevtić |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Fusarium Head Blight
0106 biological sciences Fusarium Species complex Veterinary medicine Winter wheat Trichothecene Population grain infection trichothecene genotyping 01 natural sciences lcsh:Agriculture 03 medical and health sciences chemistry.chemical_compound Clade Mycotoxin education 030304 developmental biology 0303 health sciences education.field_of_study biology lcsh:S food and beverages Alternaria biology.organism_classification winter wheat chemistry FGSC complex Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Agronomy, Vol 11, Iss 778, p 778 (2021) Agronomy Volume 11 Issue 4 |
| ISSN: | 2073-4395 |
| Popis: | Monitoring changes in the prevalence of Fusarium species and toxin production is an important tool for the integrated control of Fusarium head blight (FHB). However, methods for the high-throughput screening of Fusarium populations have been developed using isolates with limited geographic origins. In this study, we used species- and trichothecene-specific primers to monitor the F. graminearum species complex (FGSC) originating from Serbia. We also tested the applicability of the primers to the surveillance of FHB. We analyzed two hundred and ten isolates collected from thirty two locations and five winter wheat varieties over a three-year period. Using multiple correspondence analysis (MCA), we investigated associations between Fusarium-damaged kernels (FDK) and location, variety, members of the FGSC, and their predisposition for mycotoxin production. The results revealed that the species-specific primers were not specific for 11% of the F. graminearum population. The primer sets were 98.5%, 95.2%, and 92.4% effective in the multilocus genotyping of Tri7, Tri3, and Tri5 genes, respectively. We found that individual wheat varieties were associated with isolates that could not be characterized using species- and trichothecene-specific primers. Alternaria spp. had a significant influence (p < 0.001) on grain infection with F. graminearum, indicating the necessity to further investigate its impact on the pathogenesis of the F. graminearum clade. |
| Databáze: | OpenAIRE |
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