Protective DNA vaccination against experimental autoimmune encephalomyelitis is associated with induction of IFNβ

Autor: Judit Wefer, Robert A. Harris, Anna Lobell
Rok vydání: 2004
Předmět:
Central Nervous System
Lipopolysaccharides
Time Factors
T-Lymphocytes
Genetic enhancement
Cell
Major Histocompatibility Complex
Mice
Vaccines
DNA
Immunology and Allergy
Lymphocytes
Hepatitis A Virus Cellular Receptor 2
Cells
Cultured
biology
Reverse Transcriptase Polymerase Chain Reaction
Vaccination
Experimental autoimmune encephalomyelitis
Receptors
Interleukin-12
Flow Cytometry
Myelin-Associated Glycoprotein
medicine.anatomical_structure
Neurology
Antigens
Surface
Cytokines
Receptors
Virus
Myelin Proteins
NK Cell Lectin-Like Receptor Subfamily B
Encephalomyelitis
Autoimmune
Experimental
Immunology
Myelin oligodendrocyte glycoprotein
DNA vaccination
Immune system
Downregulation and upregulation
medicine
Animals
Humans
Lectins
C-Type
RNA
Messenger
Interferon-beta
Receptors
Interleukin
medicine.disease
Peptide Fragments
Rats
Disease Models
Animal
Gene Expression Regulation
Rats
Inbred Lew
biology.protein
Myelin-Oligodendrocyte Glycoprotein
Neurology (clinical)
Sequence Alignment
Zdroj: Journal of Neuroimmunology. 149:66-76
ISSN: 0165-5728
Popis: DNA vaccines encoding encephalitogenic peptides protect against subsequent development of rat experimental autoimmune encephalomyelitis (EAE) through unknown mechanisms. We investigated immune cell phenotypes at different time points after DNA vaccination with vaccine encoding myelin oligodendrocyte glycoprotein peptide 91-108 and subsequent induction of EAE. In protected rats, we observed (i) no alterations in antigen-specific Th2 or Th3 responses, (ii) reduced MHC II expression on splenocytes early after EAE induction, (iii) antigen-specific upregulation of IFNbeta upon recall stimulation and (iv) reduced IL-12Rbeta2 on lymphocytes. We suggest that the underlying mechanism of DNA vaccination is associated with immunomodulation exerted by induced IFNbeta.
Databáze: OpenAIRE
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