Application of bioluminescence imaging to therapeutic intervention of herpes simplex virus type I – Thymidine kinase/ganciclovir in glioma
| Autor: | Wee Sup Chung, Chang Woon Choi, Kwang Il Kim, Yong Jin Lee, Gi Jeong Cheon, Su Jin Jang, Chun Jeih Ryu, Hee Chung Kwon, Kyo Chul Lee, Tae Sup Lee, Joo Hyun Kang, Kwang Sun Woo, Sang Moo Lim, Tae Hyun Choi |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Ganciclovir
Cancer Research Cell Survival Recombinant Fusion Proteins Genetic Vectors Mice Nude Antineoplastic Agents Herpesvirus 1 Human Biology Thymidine Kinase Viral vector Mice Genes Reporter Luciferases Firefly Cell Line Tumor Idoxuridine Glioma medicine Animals Bioluminescence imaging Bioluminescence Luciferase Mice Inbred BALB C Dose-Response Relationship Drug Brain Neoplasms Lentivirus Genes Transgenic Suicide Genetic Therapy medicine.disease Molecular biology Rats Tumor Burden Kinetics Tumor Size Measurement Oncology Thymidine kinase Positron-Emission Tomography Luminescent Measurements Cancer research Female Glioblastoma medicine.drug |
| Zdroj: | Cancer Letters. 297:84-90 |
| ISSN: | 0304-3835 |
| DOI: | 10.1016/j.canlet.2010.04.028 |
| Popis: | Lentiviral vector containing the HSV1-tk and firefly luciferase (fLuc) gene was infected into C6 and C6-TL expressing HSV1-tk and fLuc gene was generated. C6-TL showed higher [ 125 I]IVDU uptake than C6. The survival rate of C6-TL decreased more rapidly with increasing GCV dose and was well correlated with fLuc activity. The images of microPET clearly demonstrated higher uptake of [ 18 F]FHBG into the C6-TL tumor. Inhibition of tumor growth was observed in C6-TL tumor-bearing mice treated with GCV through tumor size measurement and bioluminescence imaging. The therapeutic effect of HSV1-tk/GCV system can be monitored using bioluminescent imaging and tumor size measurement. |
| Databáze: | OpenAIRE |
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