Comparison study of the performance of the QIAGEN EGFR RGQ and EGFR pyro assays for mutation analysis in non-small cell lung cancer
| Autor: | Allison M. Cushman-Vokoun, Ann Crowley, Sharleen A. Rapp, Timothy C. Greiner |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Lung Neoplasms DNA Mutational Analysis Biology Adenocarcinoma medicine.disease_cause Real-Time Polymerase Chain Reaction Sensitivity and Specificity law.invention Cell Line Exon symbols.namesake Mutation Rate law Limit of Detection Carcinoma Non-Small-Cell Lung medicine Humans Epidermal growth factor receptor Lung cancer Polymerase chain reaction Aged Sanger sequencing Aged 80 and over Mutation Paraffin Embedding Smoking General Medicine DNA Neoplasm Sequence Analysis DNA Middle Aged medicine.disease Molecular biology ErbB Receptors Real-time polymerase chain reaction Mutation testing symbols biology.protein Female |
| Zdroj: | American journal of clinical pathology. 140(1) |
| ISSN: | 1943-7722 |
| Popis: | Objectives: To compare 2 laboratory assays commonly used in the evaluation of epidermal growth factor receptor (EGFR) mutations in non–small cell lung cancer (NSCLC). Methods: Fifty-three formalin-fixed, paraffin-embedded NSCLC specimens were selected. Extracted DNA was analyzed using the EGFR RGQ Amplification Refractory Mutation System Scorpions probe-based real-time polymerase chain reaction (PCR) assay and the EGFR Pyro pyrosequencing assay. Results: Fourteen EGFR mutations were identified in 13 specimens using at least 1 of the assays, with a mutation concordance rate of 92.9%. Using dideoxy sequencing as the gold standard, clinical sensitivity was 73.7% and 68.4% by the RGQ and Pyro assays, respectively, but 100% by both for common drug sensitivity mutations. Performance observations included the following: the RGQ system requires higher DNA input, the RGQ system is a single-step procedure, the EGFR Pyro assay is a 2-step procedure, only the RGQ system can identify exon 20 insertions, the RGQ system is more sensitive, and the Pyro system can specify exact mutations for all interrogated sites. Conclusions: Both the RGQ real-time PCR and Pyro assays adequately detect common EGFR mutations; however, the RGQ system is more clinically and analytically sensitive. Performance characteristics should be considered when evaluating these EGFR mutation assays for clinical adoption. |
| Databáze: | OpenAIRE |
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