Inhibition of Cytosolic Phospholipase A2α (cPLA2α) by Medicinal Plants in Relation to Their Phenolic Content
| Autor: | Thorsten Benz, Cornelia Zapp, Eva Arnold, Michael Wink |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Antioxidant
DPPH medicine.medical_treatment Sus scrofa Anti-Inflammatory Agents antioxidant activity Pharmaceutical Science Antioxidants Article Analytical Chemistry lcsh:QD241-441 chemistry.chemical_compound Phenols lcsh:Organic chemistry Sanguisorba officinalis Drug Discovery arachidonic acid medicine Animals Ononis spinosa Enzyme Inhibitors Physical and Theoretical Chemistry Folin–Ciocalteu reagent Urtica dioica Chromatography High Pressure Liquid anti-inflammatory Plants Medicinal Traditional medicine biology Plant Extracts Group IV Phospholipases A2 Organic Chemistry medicinalplants Orthosiphon stamineus biology.organism_classification chemistry Biochemistry Chemistry (miscellaneous) Polyphenol phenolic content Molecular Medicine cytosolic phospholipase A2α medicinal plants |
| Zdroj: | Molecules, Vol 20, Iss 8, Pp 15033-15048 (2015) Molecules Volume 20 Issue 8 Pages 15033-15048 |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules200815033 |
| Popis: | The cytosolic phospholipase A2α (cPLA2α) is one of the potential targets for anti-inflammatory drugs, since this enzyme plays a key role in the inflammation processes seen in health disorders, like asthma, allergic reactions, arthritis and neuronal diseases. In this study, cPLA2α inhibition by 43 methanol extracts from medicinal plants rich in polyphenols was determined. The eight most active extracts were derived from Ribes nigrum (IC50 of 27.7 µg/mL), Ononis spinosa (IC50 of 39.4 µg/mL), Urtica dioica (IC50 of 44.32 µg/mL), Betula sp. (IC50 of 58.02 µg/mL), Sanguisorba officinalis (IC50 of 76.25 µg/mL), Orthosiphon stamineus (IC50 of 78.83 µg/mL), Petasites hybridus (IC50 of 81.02 µg/mL) and Tussilago farfara (IC50 of 123.28 µg/mL). Additionally, the antioxidant activities of these extracts were determined with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and their phenolic content with the Folin–Ciocalteu reagent. Antioxidant activity showed a non-linear, positive correlation to the phenolic content, but no correlation of PLA2α inhibition with phenolic content could be established. This study provides evidence that cPLA2α may be a relevant target for anti-inflammatory agents. |
| Databáze: | OpenAIRE |
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