Identification of human sperm antigens reacting with antisperm antibodies from sera and genital tract secretions
| Autor: | Shafrira Shai, Yehudith Naot |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
Infertility media_common.quotation_subject Immunoblotting Fertility Biology Autoantigens Andrology Antigen Immunopathology medicine Humans Genitalia Autoantibodies media_common Autoantibody Obstetrics and Gynecology medicine.disease Spermatozoa Sperm Molecular Weight Reproductive Medicine Genital tract Immunology biology.protein Female Antibody |
| Zdroj: | Fertility and Sterility. 58:593-598 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/s0015-0282(16)55269-8 |
| Popis: | Objective To identify sperm antigens reacting with antisperm antibodies relevant in human infertility. Design The reactions of separated sperm antigens with antibodies present in sera and genital tract secretions from infertile and fertile females and males were examined by immunoblotting techniques. Setting The patients were followed in an outpatient setting of a hospital clinic. Patients One hundred consecutive infertile males and females, referred for determinations of antisperm antibodies, comprised the study group. Fifty hospital and faculty employees with proven fertility served as a control group. Results A high proportion of sera from fertile and infertile humans contained antibodies reacting with at least one sperm antigen. However, two discrete bands of antigenic proteins with molecular weights of 44 and 72 kd reacted significantly more frequently with serum antibodies from infertile females than from fertile females. No apparent correlation could be demonstrated between any particular antigen and serum antibodies from infertile males. Nevertheless, antigenic proteins of 62 kd were identified as the major sperm antigens reacting with antibodies present in seminal plasmas from infertile males. Conclusions The major sperm antigens reacting with systemic antibodies differ from the antigens recognized by local antisperm antibodies. Sperm antigens exhibiting relative molecular weights of 62 kd are major antigens reactive with local antisperm antibodies from infertile humans. |
| Databáze: | OpenAIRE |
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