Siderophore Production by Pathogenic Mucorales and Uptake of Deferoxamine B
| Autor: | Jean-Philippe Bouchara, Danielle Bomal, Bienvenue Razafimandimby, Gérald Larcher, Marylène Dias |
|---|---|
| Přispěvatelé: | Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), MOLTECH-Anjou, Université d'Angers (UA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2013 |
| Předmět: |
Electrophoresis
Mucorales Siderophore Somatic cell [SDV]Life Sciences [q-bio] Veterinary (miscellaneous) Siderophores Deferoxamine Iron chelate Applied Microbiology and Biotechnology Microbiology Fungal Proteins 03 medical and health sciences Rhizopus medicine Rhizopus arrhizus 030304 developmental biology 0303 health sciences biology 030306 microbiology Mucormycosis Metabolism biology.organism_classification medicine.disease Culture Media 3. Good health Molecular Weight Microscopy Fluorescence Biochemistry Agronomy and Crop Science |
| Zdroj: | Mycopathologia Mycopathologia, 2013, 176 (5-6), pp.319-328. ⟨10.1007/s11046-013-9693-5⟩ |
| ISSN: | 1573-0832 0301-486X |
| DOI: | 10.1007/s11046-013-9693-5 |
| Popis: | International audience; Clinical reports have established that mucormycosis, mainly caused by Rhizopus spp., frequently occurs in patients treated with deferoxamine B (DFO, Desferal®) which is misappropriated by these fungi. Siderophore production by twenty mucoralean isolates was therefore investigated using a commercial iron-depleted culture medium. Siderophore production was detected for most of the isolates. Our experiments confirmed that feroxamine B (iron chelate of DFO) promoted in vitro growth of Rhizopus arrhizus. Electrophoretic analysis of somatic extracts revealed iron-regulated proteins of 60 and 32kDa which were lacking in iron-depleted culture conditions. Using a fluorescent derivative of deferoxamine B, we showed by fluorescence microscopy the entry of the siderophore within the fungal cells, thus suggesting a shuttle mechanism encompassing the uptake of the entire siderophore-ion complex into the cell. This useful tool renders possible a better understanding of iron metabolism in Mucorales which could lead to the development of new diagnostic method or new antifungal therapy using siderophores as imaging contrast agents or active drug vectors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink