Potassium flux and sodium transport in the isolated frog skin
| Autor: | JoséA. Zadunaisky, Oscar A. Candia |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Cell Membrane Permeability
Potassium Sodium Biophysics Analytical chemistry Biological Transport Active chemistry.chemical_element Ionic bonding In Vitro Techniques Biochemistry Rana Flux (metallurgy) Sulfur Isotopes Animals Skin Rana catesbeiana integumentary system Sulfates Open-circuit voltage Cell Membrane Osmolar Concentration Inulin Cell Biology Anatomy Kinetics chemistry Permeability (electromagnetism) Potassium Isotopes Anura Extracellular Space Frog Skin |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Biomembranes. 255:517-529 |
| ISSN: | 0005-2736 |
| DOI: | 10.1016/0005-2736(72)90155-1 |
| Popis: | The K + fluxes at the inside surface, and the short-circuit current (s.c.c.), were measured in a 20-cm 2 piece of the abdominal skin of the frogs, Leptodactylus ocellatus and Rana catesbeiana . The inside of the skin was perfused with Ringer's solution containing 42 K + circulating in a closed system including a scintillation detector. The disaappearance of radioactive material from the Ringer's solution was measured during a period greater than 3 h. A compartmental analysis was used to find out the transfer constants and unidirectional fluxes. Most of the experiments were performed under steady-state conditions. Despite the fact that the skin of L. ocellatus shows an active transport of Cl − not present in the skin of the R. catesbeiana , results in both skins were similar. The K + content of the skins was found to be in the order of 1 μequiv/cm 2 of skin surface. K + influx at the inside surface was of the order of 0.3 μequiv./cm 2 per h. The K + flux did not vary when the short-circuit condition was changed to open circuit and vice versa , or upon removal of Cl − from the Ringer. However, the Na + flux, or s.c.c. was significantly larger than the K + flux. The permeability properties for K + at the inside surface seem to be relatively independent of external electrical or ionic changes. K + flux is not directly related to the mechanism of Na + transport. |
| Databáze: | OpenAIRE |
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