Choice of fixative is crucial to successful immunohistochemical detection of phosphoproteins in paraffin-embedded tumor tissues
| Autor: | Nelly Kuklin, Carol A. Cheney, Yangsi Ou, Zhiqiang Zhang, Janine A. Burns, Laura L. Franlin-Pfeifer, Yuan Li |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Cell signaling
Pathology medicine.medical_specialty Histology Lung Neoplasms Polymers Transplantation Heterologous Mice Nude Breast Neoplasms Biology Receptor tyrosine kinase Article chemistry.chemical_compound Fixatives Mice Formaldehyde Neoplasms medicine Biomarkers Tumor Animals Humans Paraformaldehyde Fixative Phosphoproteins Immunohistochemistry Staining chemistry Phosphoprotein Data Interpretation Statistical Colonic Neoplasms biology.protein Phosphorylation Female Anatomy Neoplasm Transplantation |
| Zdroj: | The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 57(3) |
| ISSN: | 0022-1554 |
| Popis: | Protein phosphorylation is frequently used as an indicator of cellular signaling activity. Elevated phosphorylation of tyrosine kinase receptors plays an important role in cancer pathogenesis. However, phosphoproteins are usually poorly preserved in clinical tissue samples that are routinely fixed in 10% formalin. Nonetheless, in oncology clinical trials, use of phosphoproteins as biomarkers has been considered to be of great value in evaluating the effectiveness of a given drug candidate. Therefore, it is worthy of investigating whether alternative fixatives would improve the preservation of phosphoproteins in tissue. We compared the IHC staining of a number of phosphoproteins in xenograft and human surgical tumor tissues fixed in three different fixatives: 10% formalin, 4% paraformaldehyde (PFA), and Streck’s tissue fixative (STF). We found that STF significantly enhanced the staining intensity of phosphoproteins compared with 10% formalin or 4% PFA. STF fixative also showed superiority of preservation of phosphoproteins in human surgical samples. Our results indicate that the choice of fixative could significantly affect the usability of clinical tissue samples for evaluating phosphoprotein by IHC. (J Histochem Cytochem 57:257–264, 2009) |
| Databáze: | OpenAIRE |
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