Molecular Characterization of Clinical Carbapenem-Resistant Enterobacteriaceae Isolates from Sétif, Algeria
| Autor: | Seydina M. Diene, Larbi Zakaria Nabti, Ahmed Olowo-Okere, Jean-Marc Rolain, David Lupande-Mwenebitu, Abderrahim Benslama, Abdenassar Harrar, Farida Sahli |
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| Přispěvatelé: | Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Klebsiella pneumoniae Immunology Carbapenem-resistant enterobacteriaceae Microbial Sensitivity Tests Biology medicine.disease_cause Microbiology beta-Lactamases Hospitals University [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system Bacterial Proteins [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Enterobacter cloacae medicine Escherichia coli Species identification Humans In patient [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology ComputingMilieux_MISCELLANEOUS Pharmacology [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases biology.organism_classification University hospital Enterobacteriaceae [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Anti-Bacterial Agents Carbapenem-Resistant Enterobacteriaceae Genes Bacterial Algeria [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
| Zdroj: | Microbial Drug Resistance Microbial Drug Resistance, 2021, ⟨10.1089/mdr.2021.0123⟩ |
| ISSN: | 1931-8448 1076-6294 |
| DOI: | 10.1089/mdr.2021.0123⟩ |
| Popis: | This study aimed to determine the incidence and the molecular mechanisms of carbapenem-resistant Enterobacteriaceae in patients from the Setif University Hospital, Algeria. Nonduplicate clinical bacterial isolates recovered from patients attending the University Hospital of Setif were collected between April and October 2018. Species identification was performed by MALDI-TOF/MS (matrix-assisted laser desorption ionization-time of flight mass spectrometry) method. The susceptibility of the isolates to carbapenems was determined using the disc diffusion method. The carbapenem resistant isolates were screened for the presence of common carbapenemase genes (blaKPC, blaOXA-48, blaVIM, blaIMP, and blaNDM) and extended-spectrum β-lactamase (blaCTX, blaTEM, and blaSHV) using PCR and sequencing technique. A total of 123 nonrepetitive Enterobacteriaceae isolates were obtained. Klebsiella pneumoniae (n = 52/42.28%), Escherichia coli (n = 24/19.51%), and Enterobacter cloacae (n = 19/15.45%) were the most prevalent species. The Carba-NP test showed that 6 out of 123 isolates carried carbapenemase enzymes. OXA-48 was found in five isolates (four K. pneumoniae and one E. coli) and NDM-5 in one E. cloacae isolate. We reported for the first time in Algeria the presence of NDM-5 carbapenemase enzyme in a clinical E. cloacae isolate. |
| Databáze: | OpenAIRE |
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