Precursor miR-499a Variant but not miR-196a2 is Associated with Rheumatoid Arthritis Susceptibility in an Egyptian Population
| Autor: | Ahmed A. Toraih, Eman A. Toraih, Manal S. Fawzy, Nesreen M. Ismail, Mohammad H. Hussein |
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| Předmět: |
0301 basic medicine
Male Risk medicine.medical_specialty Genotype Population Single-nucleotide polymorphism Bioinformatics Gastroenterology Models Biological Arthritis Rheumatoid 03 medical and health sciences Gene Frequency Internal medicine Genetics medicine Odds Ratio Rheumatoid factor Cluster Analysis Humans Genetic Predisposition to Disease education Alleles Genetic Association Studies Pharmacology education.field_of_study Base Sequence business.industry Gene Expression Profiling Heterozygote advantage Molecular Sequence Annotation General Medicine Odds ratio medicine.disease Rheumatology MicroRNAs 030104 developmental biology Rheumatoid arthritis Case-Control Studies Population Surveillance Molecular Medicine Egypt Female business |
| Zdroj: | Europe PubMed Central |
| Popis: | Rheumatoid arthritis (RA) has a complex component induced by several genes that interact together with environmental and hormonal factors. We aimed to investigate the association of miR-196a2 rs11614913 (C/T) and miR-499a rs3746444 (A/G) polymorphisms and their combination with RA susceptibility and disease activity in an Egyptian population, and to evaluate their impact on methotrexate drug response and toxicity. Bioinformatics databases were searched to select potential micro RNA (miRNA)–messenger RNA (mRNA) interactions involved in RA pathogenesis. Ninety-five RA patients diagnosed according to the American College of Rheumatology and 200 healthy controls were genotyped using real-time polymerase chain reaction technology. In overall and stratified analysis, miR-499a, but not miR-196a2, was associated with RA risk. Heterozygote carriers with rs3746444*A/G displayed protection against developing RA (p = 0.005) with an odds ratio of 0.2 (95 % confidence interval 0.17–0.62). The carriage of the combinations (miR499a*AG + miR196a2*CC) and (miR499a*AA + miR196a2*TT) were 3 and 7.5 times more likely to develop RA, respectively, while the combinations (miR499a*GG + miR196a2*CC), (miR499a*AG + miR196a2*TT) and (miR499a*AA + miR196a2*CT) show less susceptibility to have RA disease (all p |
| Databáze: | OpenAIRE |
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