Effects of in Utero PFOS Exposure on Transcriptome, Lipidome, and Function of Mouse Testis
| Autor: | Aman Yi-Man Wong, Thierry Durand, Jean-Marie Galano, Rong Li, Keng Po Lai, Ting-Fung Chan, Camille Oger, Jetty Chung-Yung Lee, H. T. Wan, Kin Sum Leung, Chris K C Wong, Cherry C Leung, Jing-Woei Li |
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| Přispěvatelé: | Hong Kong Baptist University (HKBU), The University of Hong Kong (HKU), The Chinese University of Hong Kong [Hong Kong], Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty [SDV]Life Sciences [q-bio] Biology medicine.disease_cause Transcriptome Mice 03 medical and health sciences chemistry.chemical_compound Pregnancy Tandem Mass Spectrometry Internal medicine Hydroxyeicosatetraenoic Acids Testis medicine Animals Environmental Chemistry Fluorocarbons Sperm Count Lipid metabolism General Chemistry Lipidome Eicosapentaenoic acid 030104 developmental biology Endocrinology Alkanesulfonic Acids chemistry Docosahexaenoic acid In utero Female Arachidonic acid lipids (amino acids peptides and proteins) Water Pollutants Chemical Oxidative stress |
| Zdroj: | Environmental Science and Technology Environmental Science and Technology, American Chemical Society, 2017, 51 (15), pp.8782-8794. ⟨10.1021/acs.est.7b02102⟩ |
| ISSN: | 0013-936X 1520-5851 |
| Popis: | International audience; Transcriptomic and LC-MS/MS-based targeted lipidomic analyses were conducted to identify the effects of in utero PFOS exposure on neonatal testes and its relation to testicular dysfunction in adult offspring. Pregnant mice were orally administered 0.3 and 3 μg PFOS/g body weight until term. Neonatal testes (P1) were collected for the detection of PFOS, and were subjected to omics study. Integrated pathway analyses using DAVID, KEGG, and IPA underlined the effects of PFOS exposure on lipid metabolism, oxidative stress and cell junction signaling in testes. LC-MS/MS analysis showed that the levels of adrenic acid and docosahexaenoic acid (DHA) in testes were significantly reduced in the PFOS treatment groups. A significant linear decreasing trend in eicosapentaenoic acid and DHA with PFOS concentrations was observed. Moreover, LOX-mediated 5hydroxyeicosatetraenoic acids (HETE) and 15-HETE from arachidonic acid in the testes were significantly elevated and a linear increasing trend of 15-HETE concentrations was detected with doses of PFOS. The perturbations of lipid mediators suggested that PFOS has potential negative impacts on testicular functions. Postnatal analysis of male offspring at P63 showed significant reductions in serum testosterone and epididymal sperm count. This study sheds light into the as yet unrevealed action of PFOS on lipid mediators in affecting testicular functions. |
| Databáze: | OpenAIRE |
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