Actin filament reorganisation controlled by the SCAR/WAVE complex mediates stomatal response to darkness

Autor: Kun Jiang, Bernard Genty, Alistair M. Hetherington, Fabien Monnet, Xiaobin Ou, Zaoxu Xu, Joaquim Miguel Costa, Thomas E Batstone, Jean-Charles Isner, Michael J. Deeks
Přispěvatelé: School of Biological Sciences [Bristol], University of Bristol [Bristol], Zhejiang University, Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ecophysiologie Moléculaire des Plantes (LEMP), University of Exeter, Laboratoire d'Excellence 'Maîtrise des Systèmes de Systèmes Technologiques' (Labex MS2T), Université de Technologie de Compiègne (UTC)-Centre National de la Recherche Scientifique (CNRS), European Project: HNRT‐CT‐2002‐00254,STRESSIMAGING, Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Protéines de Protection des Végétaux (PPV), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Photosynthèse & Environnement (P&E)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: New Phytologist
New Phytologist, Wiley, 2017, 215 (3), pp.1059-1067. ⟨10.1111/nph.14655⟩
New Phytologist, 2017, 215 (3), pp.1059-1067. ⟨10.1111/nph.14655⟩
The New Phytologist
Isner, J C, Xu, Z, Costa, J M, Monnet, F, Batstone, T, Ou, X, Deeks, M J, Genty, B, Jiang, K & Hetherington, A M 2017, ' Actin filament reorganisation controlled by the SCAR/WAVE complex mediates stomatal response to darkness ', New Phytologist, vol. 215, no. 3, pp. 1059-1067 . https://doi.org/10.1111/nph.14655
ISSN: 0028-646X
1469-8137
DOI: 10.1111/nph.14655⟩
Popis: Stomata respond to darkness by closing to prevent excessive water loss during the night. Although the reorganisation of actin filaments during stomatal closure is documented, the underlying mechanisms responsible for dark-induced cytoskeletal arrangement remain largely unknown. We used genetic, physiological and cell biological approaches to show that reorganisation of the actin cytoskeleton is required for dark-induced stomatal closure. The opal5 mutant does not close in response to darkness but exhibits wild-type (WT) behaviour when exposed to abscisic acid (ABA) or CaCl2. The mutation was mapped to At5g18410, encoding the PIR/SRA1/KLK subunit of the ArabidopsisSCAR/WAVE complex. Stomata of an independent allele of the PIR gene (Atpir-1) showed reduced sensitivity to darkness and F1 progenies of the cross between opal5 and Atpir-1 displayed distorted leaf trichomes, suggesting that the two mutants are allelic. Darkness induced changes in the extent of actin filament bundling in WT. These were abolished in opal5. Disruption of filamentous actin using latrunculin B or cytochalasin D restored wild-type stomatal sensitivity to darkness in opal5. Our findings suggest that the stomatal response to darkness is mediated by reorganisation of guard cell actin filaments, a process that is finely tuned by the conserved SCAR/WAVE–Arp2/3 actin regulatory module.
Databáze: OpenAIRE
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