Analytical profiling and stability evaluation of liposomal drug delivery systems: A rapid UHPLC-CAD-based approach for phospholipids in research and quality control
| Autor: | Florian Weber, Lisa Rahnfeld, Paola Luciani |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
615 Pharmacology & therapeutics
prescription drugs Quality Control Lipid composition CAD 02 engineering and technology 01 natural sciences Analytical Chemistry Drug Delivery Systems 540 Chemistry Chromatography High Pressure Liquid Phospholipids Aerosols Detection limit Active ingredient Liposome Chemistry 010401 analytical chemistry Reproducibility of Results 021001 nanoscience & nanotechnology 0104 chemical sciences Liposomes Drug delivery Biochemical engineering Charged aerosol detector 0210 nano-technology |
| Zdroj: | Weber, Florian; Rahnfeld, Lisa; Luciani, Paola (2020). Analytical profiling and stability evaluation of liposomal drug delivery systems: a rapid UHPLC-CAD-based approach for phospholipids in research and quality control. Talanta, 220, p. 121320. Elsevier 10.1016/j.talanta.2020.121320 |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2020.121320 |
| Popis: | Phospholipids and their derivatives represent a broad range of multifunctional substances used as excipients or active ingredients by different industries due to their natural origin and unique properties. A fast and reliable quantification as well as comprehensive stability evaluation are of major importance in the process of development and quality control of lipid-based systems. Therefore, the present study is focused on the development and validation of a rapid ultra-high performance liquid chromatography – charged aerosol detector based (UHPLC-CAD) method for simultaneous detection of a multitude of natural and synthetic lipids, (charged) phospholipids, lipophilic fluorescent markers and their possible degradation products. Twenty-two compounds were characterized by a strong linear response of the detector (R2 > 0.97). Moreover, remarkable limits of detection (≤10 μg mL−1) and limits of quantification (≤25 μg mL−1) associated with a consistent reproducibility were achieved for all tested molecules. The performance of the analytical method was demonstrated by analyzing the lipid composition (after different production stages and photodegradation) of both bupivacaine loaded liposomes and a Doxil®-like formulation. The newly developed method combines a rapid, comprehensive, and efficient quantification with minimal economic effort and ecologic consequences, meeting the requirements of modern analytical processes and offering a broad range of possible applications in various industrial sectors and scientific laboratories. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect