Changing expression of chloride channels during preimplantation mouse development
| Autor: | Margot L. Day, David I. Cook, Mh Johnson, Isabel A. Arnaiz |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cytoplasm
Embryology Patch-Clamp Techniques Embryonic Development Mice Inbred Strains Cell membrane Mice Endocrinology Chloride Channels medicine Animals Inner cell mass RNA Messenger Patch clamp Blastocyst Cells Cultured reproductive and urinary physiology Chemistry Cell Membrane Embryogenesis Gene Expression Regulation Developmental Obstetrics and Gynecology Trophoblast Depolarization Cell Biology Cell biology medicine.anatomical_structure Reproductive Medicine Models Animal embryonic structures Oocytes Chloride channel Female |
| Zdroj: | REPRODUCTION. 145:73-84 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/rep-12-0055 |
| Popis: | Plasma membrane chloride channels (ClCs) play important roles in a broad range of cellular processes including cell volume regulation, proliferation, and transepithelial transport, all of which are critical during preimplantation embryonic development. In this study, the molecular and functional expression of voltage-gated ClCs was analyzed throughout preimplantation development of the mouse conceptus. mRNA transcripts for allClcngenes were detected. OnlyClcn1mRNA showed differential expression in the blastocyst, being detected in the trophectoderm but not in the inner cell mass. CLCN3 protein was detected at low levels in the cytoplasm and plasma membrane in 4-cell embryos and was localized to the apical plasma membrane of the trophoblasts in the blastocyst. Whole-cell patch-clamp recordings demonstrated the presence of a DIDS-sensitive, outwardly rectifying Cl−current throughout development, with this conductance being large at the 1-cell, morula and blastocyst stages. A second DIDS-insensitive Cl−current, which was inactivated by membrane depolarization, was present in cells differentiating into the trophoblast lineage and during blastocyst expansion. Inhibition of the DIDS-sensitive current and the DIDS-insensitive current, with 9-AC, prevented blastocyst expansion. |
| Databáze: | OpenAIRE |
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