Quorum Sensing Can Be Repurposed To Promote Information Transfer between Bacteria in the Mammalian Gut
| Autor: | S. Jordan Kerns, Lynn Bry, Marika Ziesack, Suhyun Kim, Jeffrey C. Way, Pamela A. Silver, Georg K. Gerber |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Biomedical Engineering Acyl-Butyrolactones medicine.disease_cause digestive system Biochemistry Genetics and Molecular Biology (miscellaneous) 03 medical and health sciences Mice 0302 clinical medicine Bacterial Proteins Gut bacteria medicine Escherichia coli Animals Mice Inbred BALB C biology digestive oral and skin physiology food and beverages Quorum Sensing Salmonella enterica General Medicine biology.organism_classification Gut microbiome Signaling system Cell biology Gastrointestinal Microbiome Intestines Repressor Proteins Quorum sensing 030104 developmental biology Engineering communication Trans-Activators bacteria Female 030217 neurology & neurosurgery Bacteria Signal Transduction Transcription Factors |
| Zdroj: | ACS synthetic biology. 7(9) |
| ISSN: | 2161-5063 |
| Popis: | The gut microbiome is intricately involved with establishing and maintaining the health of the host. Engineering of gut microbes aims to add new functions and expand the scope of control over the gut microbiome. To create systems that can perform increasingly complex tasks in the gut, it is necessary to harness the ability of the bacteria to communicate in the gut environment. Interestingly, acyl-homoserine lactone (acyl-HSL)-mediated Gram-negative bacterial quorum sensing, a widely used mode of intercellular signaling system in nature, has not been identified in normal healthy mammalian gut. It remains unknown whether the gut bacteria that do not natively use quorum sensing can be engineered to successfully signal to other bacteria using acyl-HSLs in the gut environment. Here, we repurposed quorum sensing to create an information transfer system between native gut Escherichia coli and attenuated Salmonella enterica serovar Typhimurium. Specifically, we functionalized one species with inducible signal production and the other with signal detection and recording using genomically integrated circuits. The information transfer system demonstrated successful intra- and interspecies signaling in the murine gut. This study provides a basis for further understanding of interbacterial interactions in an otherwise hard-to-study environment as well as a basis for further investigation of the potential of acyl-HSLs as intercellular signaling molecules of engineered gut consortia. |
| Databáze: | OpenAIRE |
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