Production and Effects of Endothelin-1 in Rat Pancreatic Stellate Cells
| Autor: | Roland Reinehr, Ulrich Warskulat, Reinhard Lüthen, Dieter Häussinger, Richard Fischer, Hanne Klonowski-Stumpe |
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| Rok vydání: | 2003 |
| Předmět: |
Male
medicine.medical_specialty Endothelin A Receptor Antagonists medicine.drug_class Endocrinology Diabetes and Metabolism Enzyme-Linked Immunosorbent Assay Biology Peptides Cyclic Contractility Paracrine signalling Endocrinology Internal medicine Internal Medicine medicine Animals Calcium Signaling RNA Messenger Rats Wistar Receptor Autocrine signalling Pancreas Cells Cultured Oligonucleotide Array Sequence Analysis Endothelin-1 Hepatology Endothelins Receptor Endothelin A Receptor antagonist Immunohistochemistry Receptor Endothelin B Endothelin 1 Peptide Fragments Endothelin B Receptor Antagonists Rats Cell biology Cell culture Hepatic stellate cell Calcium |
| Zdroj: | Pancreas. 27:67-74 |
| ISSN: | 0885-3177 |
| DOI: | 10.1097/00006676-200307000-00010 |
| Popis: | Introduction: Proliferation and matrix synthesis of activated pancreatic stellate cells (PSCs) participate in the development of chronic pancreatitis. Besides other substances, endpthelin-1 (ET-1) may influence the activation process of PSCs. Until now, ET-1 has not been studied in this particular cell type. Aims: To characterize PSCs in rat pancreas with respect to expression of ET A -receptors, production of ET-1, and physiological effects induced by ET-1 during PSC activation. Methodology: Immunocytochemical and ELISA techniques and cDNA microarray analysis were used. Physiologic effects were characterized by single cell measurements of free cytosolic Ca 2+ -concentration and of PSC contractility on collagen lattices. Results: Activation of PSCs in vitro, as assessed by α-smooth muscle actin expression, was accompanied by the de novo expression of ET A -receptors and synthesis of ET- I mRNA and protein. Cytosolic Ca 2+ -concentration was increased upon ET-1 stimulation in activated but not in quiescent PSCs. Contractility of activated PSCs was significantly reduced by the selective ET A -receptor antagonist BQ123 but not by the ET B -receptor antagonist IRL-1038. Conclusions: The results suggest that ET-1 may act as a paracrine and autocrine factor for activated PSCs and may mediate contractions of activated, but not quiescent, PSCs. |
| Databáze: | OpenAIRE |
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