Glycoprotein glycans may not be necessary for the differentiation of skeletal myoblasts
| Autor: | Eric H. Ball, Anne Brickenden, George A. Cates, Elke P. Clarke, Devki Nandan, B. D. Sanwal |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Glycosylation
Cellular differentiation Mannose Myosins Mannosyltransferases Cell Line Cell Fusion Receptors Concanavalin A chemistry.chemical_compound Polysaccharides Concanavalin A Myocyte Animals RNA Messenger Glycoproteins chemistry.chemical_classification biology Muscles Glycopeptides Cell Differentiation Cell Biology Troponin Culture Media Blood chemistry Biochemistry Cell culture Mannosyltransferase activity Mutation biology.protein Glycoprotein |
| Zdroj: | Experimental cell research. 180(1) |
| ISSN: | 0014-4827 |
| Popis: | Previous work using glycosylation inhibitors has suggested that high-mannose type but not complex type oligosaccharides on the surface of cells may play a role in the differentiation of skeletal myoblasts. Earlier, we had shown that a concanavalin A-resistant mutant derived from an L6 myoblast line fails to differentiate in a medium containing 10% horse serum. Here we show that one such concanavallin A-resistant mutant (D-1) which was reported to have oligosaccharides of the type Man(3–5)G1cNAc2, shows significant fusion ability when grown in media containing 1% horse serum. Lowering the serum concentration did not alter the dolichol-phosphate mannosyltransferase activity in D-1 which remained at low levels compared to L6. The incorporation of [3H]mannose in D-1 was found to be 60% of L6 in 10% serum whereas in 1% serum the incorporation into D-1 was further reduced to 30% of L6. [3H]mannose-labeled ConA-binding proteins isolated from L6 were quantitatively and qualitatively similar in cells grown in either 10 or 1% serum. However, in D-1 cells a further decrease in the ConA-binding ability of these glycoproteins was observed. Biochemical differentiation also occurs in D-1 upon fusion in 1% serum as seen by the increase in mRNA levels of the muscle-specific markers myosin light chain and troponin T. These results suggest the high-mannose type of oligosaccharides may not be involved in myoblast differentiation. |
| Databáze: | OpenAIRE |
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