Lipoprotein subclass profiles of hyperlipidemic diabetic mice measured by nuclear magnetic resonance spectroscopy
| Autor: | Timothy J. Lyons, Samar M. Hammad, Wesley Won, Lyn Powell-Braxton, Leslie Eldridge, James D Otvos |
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| Rok vydání: | 2003 |
| Předmět: |
Male
medicine.medical_specialty Magnetic Resonance Spectroscopy Apolipoprotein B Endocrinology Diabetes and Metabolism APOBEC-1 Deaminase Lipoproteins Hyperlipidemias Familial hypercholesterolemia Biology Diabetes Mellitus Experimental chemistry.chemical_compound Mice Endocrinology Internal medicine Diabetes mellitus Cytidine Deaminase Hyperlipidemia medicine Animals Humans Particle Size Mice Knockout Triglyceride Cholesterol Cholesterol HDL nutritional and metabolic diseases medicine.disease Lipoproteins LDL Mice Inbred C57BL Disease Models Animal chemistry Receptors LDL LDL receptor biology.protein lipids (amino acids peptides and proteins) Lipoproteins HDL Lipoprotein |
| Zdroj: | Metabolism: clinical and experimental. 52(7) |
| ISSN: | 0026-0495 |
| Popis: | Dyslipidemia accelerates vascular complications of diabetes. Nuclear magnetic resonance (NMR) analysis of lipoprotein subclasses is used to evaluate a mouse model of human familial hypercholesterolemia +/- streptozotocin (STZ)-induced diabetes. A double knockout (DKO) mouse (low-density lipoprotein receptor [LDLr] -/-; apolipoprotein B [apoB] mRNA editing catalytic polypeptide-1 [Apobec1] -/-) was studied. Wild-type (WT) and DKO mice received sham or STZ injections at age 7 weeks, yielding control (WT-C, DKO-C) and diabetic (WT-D, DKO-D) groups. Fasting serum was collected when the mice were killed (age 40 weeks) for Cholestech analysis (Cholestech Corp, Hayward, CA) and NMR lipoprotein subclass profile. By Cholestech, fasting triglyceride and total cholesterol increased in DKO-C versus WT-C. Diabetes further increased total cholesterol in DKO. High-density lipoprotein cholesterol (HDL-C) was similar among all groups. NMR revealed that LDL in all groups was present in a subclass the size of large human LDL and was increased 48-fold in DKO-C versus WT-C animals, but was unaffected by diabetes. HDL was found in a subclass equivalent to large human HDL, and was similar among groups. In conclusion, NMR analysis reveals lipoprotein subclass distributions and the effects of genetic modification and diabetes in mice, but lack of particles the size of human small LDL and small HDL may limit the relevance of the present animal model to human disease. |
| Databáze: | OpenAIRE |
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