An Early Decrease in Release of Aquaporin-2 in Urinary Extracellular Vesicles After Cisplatin Treatment in Rats
| Autor: | Sayaka Oshikawa-Hori, Hiroko Sonoda, Masahiro Ikeda |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Time Factors Urinary system aquaporin-2 Renal function cisplatin exosomes aquaporin-1 Pharmacology Kidney urologic and male genital diseases Extracellular vesicles Article Rats Sprague-Dawley Extracellular Vesicles 03 medical and health sciences 0302 clinical medicine urinary extracellular vesicles exosomes aquaporin-1 aquaporin-2 cisplatin acute kidney injury medicine Animals urinary extracellular vesicles lcsh:QH301-705.5 Cisplatin Aquaporin 2 Aquaporin 1 Chemistry urogenital system Body Weight Acute kidney injury General Medicine medicine.disease Microvesicles 030104 developmental biology acute kidney injury lcsh:Biology (General) Creatinine 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Cells, Vol 8, Iss 2, p 139 (2019) Cells Volume 8 Issue 2 |
| ISSN: | 2073-4409 |
| Popis: | Aquaporin-1 (AQP1) and AQP2 are important proteins involved in the regulation of renal water handling. Both AQPs have been found in urinary extracellular vesicles (uEVs) (uEV-AQP1 and -AQP2). Cisplatin, an antineoplastic agent, is known to down-regulate renal AQP1 and AQP2. However, the effect of cisplatin on the release of uEV-AQP1 and -AQP2 is largely unknown. In this study, we examined whether treatment of rats with cisplatin affected the release of uEV-AQP1 and -AQP2. Blood tests indicated that renal function was little altered at 24 h after cisplatin treatment but thereafter decreased dramatically at all of the other time points examined. Release of uEV-AQP1 was slightly increased at 24 h and decreased at 168 h. On the other hand, release of uEV-AQP2 was decreased dramatically at 24 h, and the decrease was maintained during the experimental period. These data suggest that uEV-AQP2 can be used to detect early renal impairment due to cisplatin. Furthermore, a combination of uEV-AQP2 and -AQP1 may be useful for estimation of cisplatin-induced renal injury in a stage-dependent manner. |
| Databáze: | OpenAIRE |
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