Stereoselective determination of citalopram and desmethylcitalopram in human plasma and breast milk by liquid chromatography tandem mass spectrometry

Autor: Chantal Csajka, Delphine Grosjean, Kim An Nguyen, Chin B. Eap, Etienne Weisskopf, Alice Panchaud, Jean-Michel Hascoet, Nicolas Ansermot
Přispěvatelé: School of Pharmaceutical Sciences, University of Geneva [Switzerland], Service de Néonatalogie [Lyon], Hôpital de la Croix-Rousse [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Service de Pharmacologie Clinique (EPICIME), Hospices Civils de Lyon (HCL), CIC CHU Lyon (inserm), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Médecine Néonatale [CHRU Nancy], Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Développement, Adaptation et Handicap. Régulations cardio-respiratoires et de la motricité (DevAH), Université de Lorraine (UL), Unit of Pharmacogenetics and Clinical Psychopharmacology [Lausanne], Centre for Psychiatric Neuroscience [Lausanne], Department of Psychiatry [Lausanne], Université de Lausanne (UNIL)-Centre Hospitalier Universitaire Vaudois [Lausanne] (CHUV)-Université de Lausanne (UNIL)-Centre Hospitalier Universitaire Vaudois [Lausanne] (CHUV)-Department of Psychiatry [Lausanne], Université de Lausanne (UNIL)-Centre Hospitalier Universitaire Vaudois [Lausanne] (CHUV)-Université de Lausanne (UNIL)-Centre Hospitalier Universitaire Vaudois [Lausanne] (CHUV)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Desmethylcitalopram
Spectrometry
Mass
Electrospray Ionization
Breast milk
Electrospray ionization
Clinical Biochemistry
Pharmaceutical Science
Citalopram
Tandem mass spectrometry
030226 pharmacology & pharmacy
01 natural sciences
High-performance liquid chromatography
Analytical Chemistry
03 medical and health sciences
chemistry.chemical_compound
Plasma
0302 clinical medicine
LC–MS/MS
Liquid chromatography–mass spectrometry
Tandem Mass Spectrometry
Quantification
Drug Discovery
Humans
Solid phase extraction
Spectroscopy
Chromatography
High Pressure Liquid
Chromatography
Reverse-Phase
Chromatography
Milk
Human
Enantiomer
010401 analytical chemistry
Selected reaction monitoring
Solid Phase Extraction
Reproducibility of Results
Stereoisomerism
Reversed-phase chromatography
0104 chemical sciences
chemistry
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Zdroj: Journal of Pharmaceutical and Biomedical Analysis
Journal of Pharmaceutical and Biomedical Analysis, Elsevier, 2016, 131, pp.233-245. ⟨10.1016/j.jpba.2016.08.014⟩
ISSN: 0731-7085
Popis: International audience; A high performance liquid chromatography (HPLC) tandem mass spectrometry (MS/MS) method was developed for the simultaneous, stereoselective quantification of the antidepressant citalopram and its active metabolite desmethylcitalopram in human plasma and breast milk. Sample preparation was performed by a two-step approach, including generic protein precipitation with acetonitrile followed by solid phase extraction. Enantiospecific separation of analytes was achieved on a Phenomenex® Lux Cellulose-2 column (4.6mm×150mm; 5μm), using reversed phase chromatography conditions characterized by a gradient elution of ammonium acetate buffer (pH 9.0; 20mM) and acetonitrile at a flow rate of 0.6ml/min. The compounds were detected by a tandem quadrupole mass spectrometer equipped with an electrospray ionization source and operating in multiple reaction monitoring mode. The method was fully validated in both biological fluids over a large concentration range of 0.1-100ng/ml for S-(+)- and R-(-)-citalopram, and 0.3-100ng/ml for S-(+)- and R-(-)-desmethylcitalopram. Trueness (90.0-113.3% and 97.1-103.6%), repeatability (0.9-15.9% and 0.9-8.4%) and intermediate precision (1.3-17.8% and 0.9-9.6%) in plasma and breast milk, respectively, meet international guidelines for method validation. Internal standard-normalized matrix effects ranged between 99 and 101% and 98-105%, respectively. The accuracy profiles (total error of trueness and precision) were mostly within the acceptance limits for biological samples defined as ±30%. The method was successfully applied to patient samples in a clinical trial setting.
Databáze: OpenAIRE
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