Rapid Determination of the Amino Acid Configuration of Xenotetrapeptide
| Autor: | Helge B. Bode, Florian Fleischhacker, Friederike I. Nollmann, Edna Bode, Carsten Kegler, Tilman Ahrendt |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Stereochemistry
Molecular Conformation Peptide Mass spectrometry 01 natural sciences Biochemistry Mass Spectrometry Xenorhabdus 03 medical and health sciences Coli strain Escherichia coli Molecular Biology Chromatography High Pressure Liquid Transaminases 030304 developmental biology chemistry.chemical_classification 0303 health sciences Xenorhabdus nematophila Strain (chemistry) 010405 organic chemistry Escherichia coli Proteins Organic Chemistry Absolute configuration Deuterium 0104 chemical sciences Amino acid chemistry Molecular Medicine Hydrogen–deuterium exchange Peptides |
| Zdroj: | ChemBioChem |
| ISSN: | 1439-4227 |
| DOI: | 10.1002/cbic.201300602 |
| Popis: | An E. coli strain with deletions in five transaminases (ΔaspC ΔilvE ΔtyrB ΔavtA ΔybfQ) was constructed to be unable to degrade several amino acids. This strain was used as an expression host for the analysis of the amino acid configuration of nonribosomally synthesized peptides, including the novel peptide "xenotetrapeptide" from Xenorhabdus nematophila, by using a combination of labeling experiments and mass spectrometry. Additionally, the number of D-amino acids in the produced peptide was assigned following simple cultivation of the expression strain in D2 O. |
| Databáze: | OpenAIRE |
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