Ultrastructural Features of Human Liver Specimens from Patients Who Died of Dengue Hemorrhagic Fever
| Autor: | Nusara Chomanee, Sutee Yoksan, Ne Win, Nuntaya Punyadee, Komgrid Charngkaew, Urai Chaisri, Min Min Win, Khin Saw Aye, Panisadee Avirutnan, Prida Malasit |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
viruses 030106 microbiology lcsh:Medicine Autopsy dengue hemorrhagic fever Biology Dengue virus medicine.disease_cause liver Article 03 medical and health sciences autopsy In vivo medicine dengue virus ultrastructure electron microscope suckling mouse brain General Immunology and Microbiology Microglia Endoplasmic reticulum lcsh:R Public Health Environmental and Occupational Health Virology In vitro 030104 developmental biology Infectious Diseases medicine.anatomical_structure Viral replication Ultrastructure |
| Zdroj: | Tropical Medicine and Infectious Disease Tropical Medicine and Infectious Disease; Volume 4; Issue 2; Pages: 63 Tropical Medicine and Infectious Disease, Vol 4, Iss 2, p 63 (2019) |
| ISSN: | 2414-6366 |
| Popis: | Recent advances in electron microscopy and tomography have revealed distinct virus-induced endoplasmic reticulum (ER) structures unique for dengue virus (DV) and other flaviviruses in cell culture models, including hepatocytes. These altered ultrastructures serve as sites for viral replication. In this study, we used transmission electron microscopy to investigate whether such structures were present in the liver of fatal dengue hemorrhagic fever (DHF) autopsy cases. In parallel, electron microscopic examination of suckling mouse brains experimentally infected with DV was performed as an in vivo model of acute DV infection. Typical features of ER changes containing abundance of replicative virions were observed in neurons and microglia of DV-infected suckling mouse brains (SMB). This indicated that the in vivo DV infection could induce similar viral replication structures as previously described in the in vitro DV-infected cell model. Nevertheless, liver tissues from autopsy of patients who died of DHF showed scant changes of ER membrane structures and rare particles of virions in hepatocytes, despite overwhelming evidence for the presence of viral antigens and RNA–indicating active virus replication. Instead hepatocytes contained an abundance of steatotic vesicles and structural damages. This lack of structural changes indicative of virus replication in human hepatocytes is discussed. |
| Databáze: | OpenAIRE |
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