Korean Red Ginseng Saponin Fraction Downregulates Proinflammatory Mediators in LPS Stimulated RAW264.7 Cells and Protects Mice against Endotoxic Shock
| Autor: | Sang-Keun Kim, Hye Yoon Jeong, Taddessee Yayeh, Ji-Hoon Park, Kun-Ho Jung, Jae Youl Cho, Heun-Soo Kang, Yi-Seong Kwak, Man Hee Rhee, Yong-Bum Song, Jae-Wook Oh |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Lipopolysaccharide
Pro-inflammatory cytokines Pharmacology Biochemistry Genetics and Molecular Biology (miscellaneous) Nitric oxide Proinflammatory cytokine chemistry.chemical_compound Ginseng 20(S)-protopanaxadiol saponins medicine Endotoxic shock biology business.industry Monocyte Macrophages Panax ginseng Interleukin Articles Nitric oxide synthase medicine.anatomical_structure Complementary and alternative medicine chemistry Immunology biology.protein Tumor necrosis factor alpha business Biotechnology |
| Zdroj: | Journal of Ginseng Research |
| ISSN: | 2093-4947 1226-8453 |
| Popis: | Korean red ginseng has shown therapeutic effects for a number of disease conditions. However, little is known about the anti- inflammatory effect of Korean red ginseng saponin fraction (RGSF) in vitro and in vivo. Therefore, in this study, we showed that RGSF containing 20(S)-protopanaxadiol type saponins inhibited nitric oxide production and attenuated the release of tumor necrotic factor (TNF)-α, interleukin (IL)-6, granulocyte monocyte colony stimulating factor (GMCSF), and macrophage chemo- attractant protein-1 in lipopolysaccharide (LPS) stimulated murine macrophage RAW264.7 cells. Moreover, RGSF down-regulated the mRNA expressions of inducible nitric oxide synthase, cyclooxyginase-2, IL-1β, TNF-α, GMCSF, and IL-6. Furthermore, RGSF reduced the level of TNF-α in the serum and protected mice against LPS mediated endotoxic shock. In conclusion, these results indicated that ginsenosides from RGSF and their metabolites could be potential sources of therapeutic agents against infl ammation. |
| Databáze: | OpenAIRE |
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