Oxidative stress induces mitotic arrest by inhibiting Aurora A-involved mitotic spindle formation
| Autor: | Jing Yuan, Yuanyuan Bai, Guang-Fei Wang, Quanbin Xu, Cheng Cao, Xuan Liu, Qincai Dong |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell cycle checkpoint Aurora A kinase Mitosis Apoptosis Spindle Apparatus Polo-like kinase Biology Biochemistry 03 medical and health sciences PCNT Physiology (medical) Humans Phosphorylation Chromosome separation Aurora Kinase A Vitamin K 3 Cell Cycle Checkpoints Hydrogen Peroxide Cell biology Spindle apparatus Oxidative Stress Protein Transport HEK293 Cells 030104 developmental biology MCF-7 Cells Protein Processing Post-Translational HeLa Cells |
| Zdroj: | Free Radical Biology and Medicine. 103:177-187 |
| ISSN: | 0891-5849 |
| DOI: | 10.1016/j.freeradbiomed.2016.12.031 |
| Popis: | Oxidative stress contributes to the oxidative modification of cellular components, including lipids, proteins and DNA, and results in DNA damage, cell cycle arrest, cellular dysfunction and apoptosis. However, the mechanism underlying oxidative stress-induced mitotic abnormalities is not fully understood. In this study, we demonstrated that exogenous and endogenous reactive oxygen species (ROS) promoted mitotic arrest. Delayed formation and abnormal function of the mitotic spindle, which directly impeded mitosis and promoted abnormal chromosome separation, was responsible for ROS-induced mitotic arrest. As a key regulator of mitotic spindle assembly, Aurora A kinase was hyperphosphorylated in early mitosis under oxidative stress, which may disturb the function of Aurora A in mitotic spindle formation. Our findings identified a mechanism by which ROS regulate mitotic progression and indicated a potential molecular target for the treatment of oxidative stress-related diseases. |
| Databáze: | OpenAIRE |
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