Clusterin silencing restores myoblasts viability and down modulates the inflammatory process in osteoporotic disease
| Autor: | Chiara Greggi, Monica Celi, Augusto Orlandi, Chiara Polidoro, Elena Gasbarra, Riccardo Iundusi, Giuseppe Novelli, Sabina Pucci, M. C. Piro, Maurizio Feola, Umberto Tarantino, Francesca Mastrangeli |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Sarcopenia Osteoporosis marker lcsh:Medicine Osteoarthritis Hip Histones Myoblasts 0302 clinical medicine Clusterin (CLU) Medicine Myocyte Muscle waist Aged 80 and over biology Muscle cell differentiation Acetylation General Medicine Recombinant Proteins 030220 oncology & carcinogenesis Female Myogenin medicine.symptom Adult Senescence Cell Survival CX3C Chemokine Receptor 1 Inflammation General Biochemistry Genetics and Molecular Biology Histone H4 03 medical and health sciences Osteoarthritis Humans Gene silencing Gene Silencing Muscle Skeletal Aged Cell Proliferation Clusterin Interleukin-6 business.industry Research lcsh:R DNA 030104 developmental biology Settore MED/03 - Genetica Medica Cancer research biology.protein Osteoporosis business |
| Zdroj: | Journal of Translational Medicine Journal of Translational Medicine, Vol 17, Iss 1, Pp 1-16 (2019) |
| ISSN: | 1479-5876 |
| Popis: | Background Targeting new molecular pathways leading to Osteoporosis (OP) and Osteoarthritis (OA) is a hot topic for drug discovery. Clusterin (CLU) is a glycoprotein involved in inflammation, proliferation, cell death, neoplastic disease, Alzheimer disease and aging. The present study focuses on the expression and the role of CLU in influencing the decrease of muscle mass and fiber senescence in OP-OA condition. Methods Vastus lateralis muscle biopsies were collected from 20 women with OP undergoing surgery for fragility hip fracture and 20 women undergoing arthroplasty for hip osteoarthritis. Results We found an overexpression of CLU in degenerated fibers in OP closely correlated with interleukin 6 (IL6) and histone H4 acetylation level. Conversely, in OA muscle tissues we observed a weak expression of CLU but no nuclear histone H4 acetylation. Ex vivo studies on isolated human myoblasts confirmed CLU overexpression in OP as compared to OA (p |
| Databáze: | OpenAIRE |
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