Suppression of PmRab7 by dsRNA Inhibits WSSV or YHV Infection in Shrimp
| Autor: | Kallaya Sritunyalucksana, Sakol Panyim, Mayuree Chanasakulniyom, Chalermporn Ongvarrasopone |
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| Rok vydání: | 2008 |
| Předmět: |
biology
viruses White spot syndrome rab7 GTP-Binding Proteins DNA virus Roniviridae Virus Replication biology.organism_classification Applied Microbiology and Biotechnology Virology Virus Shrimp Penaeus monodon RNA silencing White spot syndrome virus 1 Penaeidae Viral replication rab GTP-Binding Proteins Viral entry Animals RNA Interference RNA Double-Stranded |
| Zdroj: | Marine Biotechnology. 10:374-381 |
| ISSN: | 1436-2236 1436-2228 |
| Popis: | Viral entry into host cells requires endocytosis machineries of the host for viral replication. PmRab7, a Penaeus monodon small GTPase protein, was investigated for its function in vesicular transport during viral infection. The double-stranded RNA of Rab7 was injected into a juvenile shrimp before challenging with white spot syndrome virus (WSSV) or yellow head virus (YHV). PmRab7 mRNA was specifically decreased at 48 h after dsRNA-Rab7 injection. Silencing of PmRab7 dramatically inhibited WSSV-VP28 mRNA and protein expression. Unexpectedly, the silencing of PmRab7 also inhibited YHV replication in the YHV-infected shrimp. These results suggested that PmRab7 is a common cellular factor required for WSSV or YHV replication in shrimp. Because PmRab7 should function in the endosomal trafficking pathway, its silencing prevents successful viral trafficking necessary for replication. Silencing of PmRab7 could be a novel approach to prevent both DNA virus (WSSV) and RNA virus (YHV) infection of shrimp. |
| Databáze: | OpenAIRE |
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