Acetyl zingerone: An efficacious multifunctional ingredient for continued protection against ongoing DNA damage in melanocytes after sun exposure ends
| Autor: | Thomas A. Meyer, Douglas E. Brash, R.K. Chaudhuri, Sanjay Premi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Zingerone
Aging antioxidant Antioxidant DNA damage medicine.medical_treatment Pharmaceutical Science Pyrimidine dimer Dermatology quencher 030226 pharmacology & pharmacy Mice 030207 dermatology & venereal diseases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Colloid and Surface Chemistry Drug Discovery medicine Animals scavenger Cells Cultured ultraviolet radiation (UVR) chemistry.chemical_classification Reactive oxygen species dark cyclobutane pyrimidine dimers (dark‐CPDs) Chemistry Singlet oxygen Guaiacol Environmental Exposure Original Articles Molecular biology Mice Inbred C57BL Pyrimidine Dimers Chemistry (miscellaneous) Photoprotection Sunlight Melanocytes Original Article Acetyl zingerone Peroxynitrite DNA Damage |
| Zdroj: | International Journal of Cosmetic Science |
| ISSN: | 1468-2494 0142-5463 |
| Popis: | Objective Recent research has shown that significant levels of cyclobutane pyrimidine dimers (CPDs) in DNA continue to form in melanocytes for several hours in the dark after exposure to ultraviolet radiation (UVR) ends. We document the utility of a new multifunctional ingredient, 3‐(4‐hydroxy, 3‐methoxybenzyl)‐pentane‐2,4‐dione (INCI acetyl zingerone (AZ)), to protect melanocytes against CPD formation after UVR exposure ends. Methods The use of AZ as an intervention to reduce CPD formation after irradiation was assessed in vitro by comparing kinetic profiles of CPD formation for several hours after irradiation in cells that were untreated or treated with AZ immediately after irradiation. Multifunctional performance of AZ as an antioxidant, quencher and scavenger was established using industry‐standard in vitro chemical assays, and then, its efficacy in a more biological assay was confirmed by its in vitro ability to reduce intracellular levels of reactive oxygen species (ROS) in keratinocytes exposed to UVA radiation. Molecular photostability was assessed in solution during exposure to solar‐simulated UVR and compared with the conventional antioxidant α‐tocopherol. Results Even when added immediately after irradiation, AZ significantly inhibited ongoing formation of CPDs in melanocytes after exposure to UVA. Incubation with AZ before irradiation decreased intracellular levels of UVA‐induced ROS formation in keratinocytes. Compared with α‐tocopherol, the molecular structure of AZ endows it with significantly better photostability and efficacy to neutralize free radicals (∙OH, ∙OOH), physically quench singlet oxygen (1O2) and scavenge peroxynitrite (ONOO−). Conclusion These results designate AZ as a new type of multifunctional ingredient with strong potential to extend photoprotection of traditional sunscreens and daily skincare products over the first few hours after sun exposure ends. Acetyl zingerone (AZ) significantly inhibited ongoing formation of CPDs in melanocytes after exposure to UVA when added immediately after irradiation. Compared with α‐tocopherol, the molecular structure of AZ endows it with significantly better photostability and efficacy to neutralize free radicals (∙OH, ∙OOH), physically quench singlet oxygen (1O2) and scavenge peroxynitrite (ONOO−). These results designate AZ as a new type of multifunctional ingredient with strong potential to extend photoprotection over the first few hours after sun exposure ends and is highly suitable for inclusion into traditional sunscreen and daily skincare products. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text