Lipase Activity among Bacteria Isolated from Amazonian Soils
| Autor: | Aloisio Freitas Chagas, Mariana Gomes Germano, Luiz Antonio de Oliveira, Francisco Wesen Moreira, André Luis Willerding |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
4 Nitrophenylpalmitate
Article Subject Burkholderia Microflora Amazonian Enzyme Assay Palmitic Acid Derivative Bacterium Isolation Biochemistry law.invention Enzyme Activity lcsh:Biochemistry Unclassified Drug Bacterial Strain Biotransformation law Pseudomonas Flora (microbiology) Bioassay lcsh:QD415-436 Food science Lipase lcsh:QH301-705.5 Molecular Biology Petri Dish biology business.industry Brasil Petri dish Temperature Nonhuman biology.organism_classification Soil Microflora Biotechnology lcsh:Biology (General) Soil water Priority Journal biology.protein Triacylglycerol Lipase Qualitative Analysis Enzyme Synthesis business Bacteria Research Article |
| Zdroj: | Enzyme Research Enzyme Research, Vol 2011 (2011) Repositório Institucional do INPA Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
| ISSN: | 2090-0414 2090-0406 |
| Popis: | The objective of this study was to select lipase-producing bacteria collected from different counties of the Amazon region. Of the 440 bacteria strains, 181 were selected for the lipase assay in qualitative tests at Petri dishes, being 75 (41%) lipase positive. The enzymatic index was determined during fifteen days at different temperatures (30°, 35°, 40°, and 45°C). The highest lipase activity was observed within 72 hours at 30°C. Twelve bacteria strains presented an index equal to or greater than the standard used like reference, demonstrating the potential of microbial resource. After the bioassay in Petri dishes, the selected bacteria strains were analyzed in quantitative tests on p-nitrophenyl palmitate (p-NPP). A group of the strains was selected for other phases of study with the use in oleaginous substrates of the Amazonian flora, aiming for the application in processes like oil biotransformation. |
| Databáze: | OpenAIRE |
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