Histomorphometric and vascular changes in equine endometrium after the infusion of conceptus fragments
| Autor: | Cesar Augusto Camacho, Rodrigo Costa Mattos, Jorge Emilio Caballeros, N. Cazales, María José Estradé, Adriana Pires Neves, Sandra Mara Fiala-Rechsteiner |
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| Rok vydání: | 2020 |
| Předmět: |
endocrine system
media_common.quotation_subject Uterus Lumen (anatomy) Endometrium Palpation Andrology 03 medical and health sciences 0302 clinical medicine vascularization Medicine Conceptus Ovulation reproductive and urinary physiology media_common Estrous cycle 030219 obstetrics & reproductive medicine General Veterinary medicine.diagnostic_test uterus business.industry urogenital system 0402 animal and dairy science Histology 04 agricultural and veterinary sciences 040201 dairy & animal science ultrastructure histological medicine.anatomical_structure Animal Science and Zoology Original Article sense organs business |
| Zdroj: | Animal Reproduction Animal Reproduction, Volume: 17, Issue: 2, Article number: e20200006, Published: 08 JUL 2020 |
| ISSN: | 1984-3143 |
| Popis: | This experiment aimed to verify if the proteins present in a 13th day conceptus induce changes in the equine endometrial ultra-structure, histology, and vascularization, two days after its infusion. Ten healthy cyclic mares were used. Once estrus was confirmed, mares were examined daily to detect ovulation (day 0). After ovulation, mares were examined daily until day seven by transrectal palpation and B-mode and Doppler ultrasonography. In this first cycle, intrauterine biopsies were collected at day seven after ovulation, constituting the Cyclic group (n = 10). In the second cycle, the same mares daily were examined until ovulation was detected. After ovulation, mares were examined daily by transrectal palpation and B-mode and Doppler ultrasonography until day 7. On day 5, after ovulation, fragments from previously collected 13-day-old concepti were infused into the uterus of each mare. Intrauterine biopsies were collected at day 7 in all mares (n = 10), constituting the Fragment group. The percentage of ciliated and flattened cells decreased in the Fragment group. Protruded cells, superficial and intraglandular secretion, glandular lumen and diameter, blood vessel diameter, endometrial vascularization, and immune cells were higher in the Fragment group than in the Cyclic group. In summary, proteins of 13th day equine conceptus fragments infused at day five after ovulation signaled histological and vascular changes in the endometrium at the 7th day after ovulation. |
| Databáze: | OpenAIRE |
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