NONOates–Polyethylenimine Hydrogel for Controlled Nitric Oxide Release and Cell Proliferation Modulation
| Autor: | Hyunwoo Kim, Dong Keun Han, Yanggy Lee, Jae Ho Shin, Kaushik Singha, Jihoon Kim, Won Jong Kim, Seongbong Jo |
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| Rok vydání: | 2011 |
| Předmět: |
Models
Molecular Vascular smooth muscle Myocytes Smooth Muscle Biomedical Engineering Pharmaceutical Science Bioengineering Poloxamer Nitric Oxide Hydrogel Polyethylene Glycol Dimethacrylate Nitric oxide Structure-Activity Relationship chemistry.chemical_compound Humans Polyethyleneimine Myocyte Cells Cultured Cell Proliferation Pharmacology Polyethylenimine Molecular Structure Cell growth Organic Chemistry Endothelial Cells Controlled release Endothelial stem cell chemistry Biochemistry Cell culture Biophysics Azo Compounds Biotechnology |
| Zdroj: | Bioconjugate Chemistry. 22:1031-1038 |
| ISSN: | 1520-4812 1043-1802 |
| Popis: | In recent years, numerous research activities have been devoted to the controlled release of nitric oxide (NO) due to its potential as a restenosis inhibitor which inhibits the proliferation of vascular smooth muscle cells, the apoptosis of vascular endothelial cells, and aggregation of platelets. This work has demonstrated the development of a novel NO-conjugated gel system comprising of thermosensitive Pluronic F127, branched polyethylenimine (BPEI), and diazeniumdiolates (NONOates). Synthesis of conjugated Pluronic-BPEI-NONOates involved coupling of activated F127 to BPEI followed by the installation of NONOates at the secondary amine sites of branched PEI backbone under high pressure. NO-conjugated gel system, F127-BPEI-NONOates, reduced the initial burst of NO release and prolonged NO release. Furthermore, F127-BPEI-NONOates polymer coated on cell culture dish displayed much higher increase of endothelial cell proliferation and reduction of smooth muscle cell proliferation than that exhibited by non-NO releasing control. Such an NO-releasing device can operate locally and has a great potential in several biomedical applications due to high biocompatibility imparted by the conjugated F127. |
| Databáze: | OpenAIRE |
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