Real-Time Label-Free Embolus Detection Using In Vivo Photoacoustic Flow Cytometry
| Autor: | Alexander V. Melerzanov, Dmitry A. Nedosekin, Tareq A. Juratli, Vladimir P. Zharov, James Y. Suen, Ekaterina I. Galanzha, Mustafa Sarimollaoglu, Yulian A. Menyaev, Eric R. Siegel, Mazen A. Juratli |
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| Rok vydání: | 2015 |
| Předmět: |
Melanomas
Pathology Cardiovascular Procedures medicine.medical_treatment Embolism Melanoma Experimental Cancer Treatment lcsh:Medicine 030204 cardiovascular system & hematology Embolectomy 01 natural sciences Mice 0302 clinical medicine Medicine and Health Sciences lcsh:Science Early Detection of Cancer Multidisciplinary medicine.diagnostic_test Melanoma Animal Models Flow Cytometry 3. Good health Molecular Imaging Surgical Oncology Oncology Optical Equipment Cauterization cardiovascular system Engineering and Technology Radiology Anatomy Research Article Clinical Oncology medicine.medical_specialty Mice Nude Equipment Mouse Models Surgical and Invasive Medical Procedures Research and Analysis Methods Extracorporeal Flow cytometry 010309 optics Photoacoustic Techniques 03 medical and health sciences Model Organisms Embolus In vivo 0103 physical sciences medicine Animals ddc:610 cardiovascular diseases Surgical Excision business.industry Lasers lcsh:R Cancer Cancers and Neoplasms Biology and Life Sciences medicine.disease Transplantation Disease Models Animal Ears lcsh:Q Clinical Medicine business Head |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 5, p e0156269 (2016) |
| ISSN: | 1932-6203 |
| Popis: | Thromboembolic events are one of the world's leading causes of death among patients. Embolus or clot formations have several etiologies including paraneoplastic, post-surgery, cauterization, transplantation, or extracorporeal circuits. Despite its medical significance, little progress has been made in early embolus detection, screening and control. The aim of our study is to test the utility of the in vivo photoacoustic (PA) flow cytometry (PAFC) technique for non-invasive embolus detection in real-time. Using in vivo PAFC, emboli were non-invasively monitored in the bloodstream of two different mouse models. The tumor-free mouse model consisted of two groups, one in which the limbs were clamped to produce vessel stasis (7 procedures), and one where the mice underwent surgery (7 procedures). The melanoma-bearing mouse model also consisted of two groups, one in which the implanted tumor underwent compression (8 procedures), and one where a surgical excision of the implanted tumor was performed (8 procedures). We demonstrated that the PAFC can detect a single embolus, and has the ability to distinguish between erythrocyte-rich (red) and leukocyte/platelet-rich (white) emboli in small vessels. We show that, in tumor-bearing mice, the level of circulating emboli was increased compared to tumor-free mice (p = 0.0013). The number of circulating emboli temporarily increased in the tumor-free control mice during vessel stasis (p = 0.033) and after surgical excisions (signed-rank p = 0.031). Similar observations were noted during tumor compression (p = 0.013) and after tumor excisions (p = 0.012). For the first time, it was possible to detect unlabeled emboli in vivo non-invasively, and to confirm the presence of pigmented tumor cells within circulating emboli. The insight on embolus dynamics during cancer progression and medical procedures highlight the clinical potential of PAFC for early detection of cancer and surgery-induced emboli to prevent the fatal thromboembolic complications by well-timed therapy. |
| Databáze: | OpenAIRE |
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