A cytogenetic study of three parasitic wasp species (Hymenoptera, Chalcidoidea, Eulophidae, Trichogrammatidae) from Brazil using chromosome morphometrics and base-specific fluorochrome staining

Autor: Marco Antonio Costa, Vladimir E. Gokhman, Fabricio Fagundes Pereira
Rok vydání: 2017
Předmět:
0106 biological sciences
0301 basic medicine
Insecta
Kulbastavia
Plant Science
Carbotriplurida
01 natural sciences
Chalcid wasp
DAPI
ChalcidoideaAnimalia
Bilateria
Pterygota
Eulophidae
biology
Parasitoids
Karyotype
karyotypes
Trichogrammatidae
Circumscriptional names
Boltonocostidae
ChalcidoideaChalcidoideaCephalornis
Tiphiinae
Circumscriptional name
Ploidy
Research Article
Biotechnology
Coelenterata
Chalcidoidea
chromosomes
lcsh:QH426-470
Arthropoda
Hymenopterida
Nephrozoa
Zoology
Protostomia
Basal
010603 evolutionary biology
Circumscriptional names of the taxon under
03 medical and health sciences
Botany
Genetics
Animalia
Eumetabola
EulophidaeEulophidaeAnimalia
NORs
Morphometrics
HymenopteraAnimalia
Chromosome
biology.organism_classification
Strashila incredibilis
Hymenoptera
lcsh:Genetics
030104 developmental biology
Notchia
CMA3
Ecdysozoa
Animal Science and Zoology
Pterygota (plant)
Zdroj: Comparative Cytogenetics
Comparative Cytogenetics 11(1): 179-188
Comparative Cytogenetics, Vol 11, Iss 1, Pp 179-188 (2017)
Popis: Chromosomes of three chalcid wasp species from Brazil, Palmistichus elaeisis Delvare et LaSalle, 1993, Trichospilus diatraeae Cherian et Margabandhu, 1942 (both belonging to the family Eulophidae) and Trichogramma pretiosum Riley, 1879 (Trichogrammatidae), were studied using chromosome morphometrics and base-specific fluorochrome staining. The present study confirmed that these species respectively have 2n = 12, 14 and 10. Chromomycin A3 / 4', 6-diamidino-2-phenylindole (CMA3/DAPI) staining revealed a single CMA3-positive and DAPI-negative band within haploid karyotypes of both P. elaeisis and T. pretiosum. This CG-rich band clearly corresponds to the nucleolus organizing region (NOR). Moreover, analogous multiple telomeric bands found on all chromosomes of T. diatraeae may also represent NORs. Certain features of karyotype evolution of the phylogenetic lineage comprising both Eulophidae and Trichogrammatidae are discussed. The results obtained during the present study demonstrate the importance of chromosome research on tropical parasitoids that remain poorly known in this respect.
Databáze: OpenAIRE
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