A re-investigation of the path of carbon in photosynthesis utilizing GC/MS methodology. Unequivocal verification of the participation of octulose phosphates in the pathway
| Autor: | Ian L. Flanigan, John K. MacLeod, John F. Williams |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Mox-TMS derivatives
Chloroplasts 13CO2 incorporation chemistry.chemical_element Plant Science Photosynthesis Biochemistry Gas Chromatography-Mass Spectrometry chemistry.chemical_compound Gas chromatography–mass spectrometry Spinacia oleracea Ketoses Regular Paper Derivatization Selected ion monitoring chemistry.chemical_classification Carbon Isotopes Sugar phosphates Quenching (fluorescence) Chromatography 13C-enriched sugar phosphates Ketose General Medicine Cell Biology Modified Calvin cycle Phosphate Carbon Oxygen Plant Leaves chemistry Octulose phosphate shunt Isotopes of carbon Calibration Sugar Phosphates |
| Zdroj: | Photosynthesis Research |
| ISSN: | 1573-5079 0166-8595 |
| Popis: | A GC/EIMS/SIM methodology has been developed to re-examine the path of carbon in photosynthesis. Exposing isolated spinach chloroplasts to 13CO2 on a solid support for a defined period followed by quenching and work-up provided a mixture of labelled sugar phosphates. After enzymatic dephosphorylation and derivatization, the Mox-TMS sugars were analysed using the above method. The purpose of the study was to try to calculate the atom% enrichment of 13C in as many of the individual carbons in each of the derivatized sugars as was practical using diagnostic fragment ions. In the event, only one 45 s experiment provided sufficient data to enable a range of enrichment values to be calculated. This confirmed that D-glycero-D-altro-octulose phosphate was present in the chloroplasts and was heavily labelled in the C4, C5 and C6 positions, in keeping with the hypothesis that it had an inclusive role and a labelling pattern consistent with a new modified pathway of carbon in photosynthesis. |
| Databáze: | OpenAIRE |
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